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| Status |
Public on Mar 01, 2010 |
| Title |
Differences between CD44+/CD24- and CD44-/CD24+ subpopulation of immortalized human mammary epithelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
CD44+/CD24- subpopulation of normal and cancerous breast epithelial cells are suggested to have stem cell properties. The goal of this study was to identify gene expression differences between CD44+/CD24- and CD44-/CD24+ subpopulation of cells from a same cell lines. We selected MCF-10A cells, which are immortalized derived from a fibrocystic breast disease. These cells are immortalized but not transformed and express basal cell markers.
Cells were from a single sort but plated into four 100 mm plates. RNA was prepared from each plate separately for the analysis.
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| |
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| Overall design |
Comparison of gene expression between 2 groups ( CD44+/CD24- and CD44-/CD24+) 4 replicates each.
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| |
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| Contributor(s) |
Nakshatri H, Bhat-Nakshatri P, Edenberg HJ |
| Citation(s) |
20691079 |
| |
| Submission date |
Mar 11, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Harikrishna Nakshatri |
| Organization name |
Indiana University School of Medicine
|
| Department |
General Surgery
|
| Street address |
980 W. Walnut St. R3C218C
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA116411 |
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