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| Status |
Public on May 22, 2021 |
| Title |
Structure, composition and evolution of the nonvital B chromosome of maize |
| Organism |
Zea mays |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To determine the centromere of the maize B chromosome, we used previously published anti-CENH3-ChIP-seq data from TB-9Sb, which contain a complete functional B centromere. Distribution of centromere-specific DNA repeats, including CentC, CRM element and B-repeat, were observed in the proximal end of the assembled maize B chromosome, and this region was shown to be associated with CENH3 nucleosomes. Furthermore, six small scaffolds with sizes ranging from 10 to 174 kilobase display CENH3 enrichment, also with the distribution of these repeat sequences. These results were consistent with previously cytogenetic observation. Therefore, approximately 520 kb centromeric regions were determined in the assembled maize B chromosome.
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| Overall design |
MNase-seq was carried out using material from young leaves of B73 +2B.
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*This dataset consists of 1 control sample only
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| Contributor(s) |
Han F, Birchler JA |
| Citation(s) |
34088847 |
| Submission date |
Jun 09, 2020 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Handong Su |
| E-mail(s) |
shandong@mail.hzau.edu.cn
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| Phone |
+86-18810795796
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| Organization name |
Huazhong Agricultural University
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| Street address |
No.1,Shizishan Street ยท Hongshan District
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA638269 |
| SRA |
SRP266572 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE152074_RAW.tar |
468.8 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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