|
| Status |
Public on Oct 01, 2021 |
| Title |
Cis-regulatory architecture of human ESC-derived hypothalamic neuron differentiation aids in variant-to-gene mapping of relevant common complex traits (RNA-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the role of the hypothalamus in influencing complex traits, we generated ATAC-seq, RNA-seq, and Promoter Focused Capture C to gain insight into the gene regulatory arcitecture contacting promoters in an embryonic stem cell dervived model of hypothalamic neuron differentiation
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| |
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| Overall design |
We performed ATAC-seq, RNA-seq, and Promoter Focused Capture C on stages of ESC dervived hypothalamic like neuron differentiation (ESC: day 0; HP: day 12; HN: day 27).
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| |
|
| Contributor(s) |
Pahl MC, . Grant SA |
| Citation(s) |
34799566 |
| |
| Submission date |
Jun 09, 2020 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Matthew C Pahl |
| E-mail(s) |
pahlm@email.chop.edu
|
| Phone |
5704282303
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Center for Spatial and Functional Genomics
|
| Lab |
Struan Grant and Andrew Wells
|
| Street address |
3615 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (9)
|
| GSM4603038 |
Embryonic Stem Cells (RNA-Seq) Replicate 1 |
| GSM4603039 |
Embryonic Stem Cells (RNA-Seq) Replicate 2 |
| GSM4603040 |
Embryonic Stem Cells (RNA-Seq) Replicate 3 |
| GSM4603041 |
Embryonic Stem Cell dervived Hypothalamic Progenitor-like Cells (RNA-Seq) Replicate 1 |
| GSM4603042 |
Embryonic Stem Cell dervived Hypothalamic Progenitor-like Cells (RNA-Seq) Replicate 2 |
| GSM4603043 |
Embryonic Stem Cell dervived Hypothalamic Progenitor-like Cells (RNA-Seq) Replicate 3 |
| GSM4603044 |
Embryonic Stem Cell dervived Hypothalamus-like Neurons (RNA-Seq) Replicate 1 |
| GSM4603045 |
Embryonic Stem Cell dervived Hypothalamus-like Neurons (RNA-Seq) Replicate 2 |
| GSM4603046 |
Embryonic Stem Cell dervived Hypothalamus-like Neurons (RNA-Seq) Replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE152098 |
Cis-regulatory architecture of human ESC-derived hypothalamic neuron differentiation aids in variant-to-gene mapping of relevant common complex traits |
|
| Relations |
| BioProject |
PRJNA638301 |
| SRA |
SRP266594 |
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