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| Status |
Public on Dec 31, 2021 |
| Title |
ATAC sequencing of zebrafish pancreatic beta-cells |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Pancreatic beta-calls are responsible for regulating the blood glucose levels via secretion of hormone insulin. To elucidate the chromatin state in zebrafish beta-cells, we performed ATAC-Sequencing.
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| Overall design |
Two replicates of beta-cells. For control, one replicate of hepatocytes was generated. ATAC-Seq was performed on 50k FACS sorted cells obtained from 3 mpf animals. For beta-cells, Tg(ins:Brainbow1.0) was used, while for hepatocytes, Tg(fabp10:dsRed, ela3l:GFP)gz12, also known as 2CLIP (2-color liver pancreas), was used.
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| Contributor(s) |
Singh SP |
| Citation(s) |
35088828 |
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| Submission date |
Jun 10, 2020 |
| Last update date |
Apr 01, 2022 |
| Contact name |
Sumeet Pal Singh |
| E-mail(s) |
sumeetpalsingh@gmail.com
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| Phone |
+32-473-125019
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| Organization name |
ULB
|
| Department |
IRIBHM
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| Lab |
Regeneration and Stress Biology
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| Street address |
Route de Lennik 808, Anderlecht
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| City |
Brussels |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA638621 |
| SRA |
SRP266784 |
