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Series GSE152299 Query DataSets for GSE152299
Status Public on Jan 31, 2021
Title Deciphering the molecular alterations sustaining an inverted apicobasal polarity phenotype in colorectal cancer clusters (Tumour Spheres with Inverted Polarity, TSIPs)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Colorectal cancer (CRC) is the second deadliest form of cancer and most of patients die from their metastases. As previously shown by our team, in serrated CRC patients, metastases are due to spherical clusters of epithelial cancer cells displaying an inverted apicobasal polarity. We called them TSIPs for Tumor spheres with inverted polarity.
In this study, we investigated the alterations sustaining this remarkable phenotype using PDXs and organoids techniques. In particular, we compared a model that respond normally to its microenvironment (mainly 3D collagen-I networks) to two models that keep their inverted polarity. A whole transcriptome investigation allowed us to better understand the mechanisms of such a topology.
 
Overall design Each CRC PDX model was processed in triplicates in two culture conditions: in suspension or in a 3D collagen-I network. On each biological triplicate for the three models, total RNA was processed to perform a whole transcriptome experiment by Clariom S human microarray.
 
Contributor(s) Cartry J, Canet-Jourdan C, Jaulin F, Desterke C
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Submission date Jun 11, 2020
Last update date Feb 01, 2021
Contact name christophe desterke
E-mail(s) christophe.desterke@inserm.fr
Phone +33 (0)145595316
Organization name university Paris Sud11
Department UFR medecine institute Andre Lwoff
Lab hopital Paul Brousse Batiment Lavoisier
Street address 14-16 avenue Paul Vaillant Couturier
City VILLEJUIF
ZIP/Postal code 94807
Country France
 
Platforms (1)
GPL23159 [Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay)
Samples (18)
GSM4611915 01_9C1_coll
GSM4611916 02_9C2_coll
GSM4611917 03_9C3_coll
Relations
BioProject PRJNA638924

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152299_RAW.tar 19.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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