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Status |
Public on Jan 31, 2021 |
Title |
Deciphering the molecular alterations sustaining an inverted apicobasal polarity phenotype in colorectal cancer clusters (Tumour Spheres with Inverted Polarity, TSIPs) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Colorectal cancer (CRC) is the second deadliest form of cancer and most of patients die from their metastases. As previously shown by our team, in serrated CRC patients, metastases are due to spherical clusters of epithelial cancer cells displaying an inverted apicobasal polarity. We called them TSIPs for Tumor spheres with inverted polarity. In this study, we investigated the alterations sustaining this remarkable phenotype using PDXs and organoids techniques. In particular, we compared a model that respond normally to its microenvironment (mainly 3D collagen-I networks) to two models that keep their inverted polarity. A whole transcriptome investigation allowed us to better understand the mechanisms of such a topology.
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Overall design |
Each CRC PDX model was processed in triplicates in two culture conditions: in suspension or in a 3D collagen-I network. On each biological triplicate for the three models, total RNA was processed to perform a whole transcriptome experiment by Clariom S human microarray.
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Contributor(s) |
Cartry J, Canet-Jourdan C, Jaulin F, Desterke C |
Citation missing |
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Submission date |
Jun 11, 2020 |
Last update date |
Feb 01, 2021 |
Contact name |
christophe desterke |
E-mail(s) |
christophe.desterke@inserm.fr
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Phone |
+33 (0)145595316
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Organization name |
university Paris Sud11
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Department |
UFR medecine institute Andre Lwoff
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Lab |
hopital Paul Brousse Batiment Lavoisier
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Street address |
14-16 avenue Paul Vaillant Couturier
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City |
VILLEJUIF |
ZIP/Postal code |
94807 |
Country |
France |
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Platforms (1) |
GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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Samples (18)
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Relations |
BioProject |
PRJNA638924 |