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| Status |
Public on Jun 13, 2020 |
| Title |
RNA-Sequencing Reveals the Changes of Gene Expression Profile in the Lipopolysaccharide-induced Immunoresponsed Uterine Body after the Immunosuppressant Cyclosporine A-treatment |
| Organism |
Oryctolagus cuniculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Infection of Gram-negative bacteria in female genital tract causes systematic immune-response and reproductive dysfunction. Immunosuppressor Cyclosporine A (CsA) is widely used in the treatment of genital tract immune response, however, the knowledge on the changes of transcriptome after CsA inhibiting lipopolysaccharide (LPS)-induced immune response still remains unknown.
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| Overall design |
Twelve New Zealand white rabbits (n=12) were randomly divided into 4 groups. The rabbits in the control group were treated with normal saline, and CsA solvent was intramuscularly injected. The rabbits from LPS group were treated with LPS by intrauterine infusion, and 500 μL of CsA solvent was intramuscularly injected. In the CsA treatment group, the uterus was perfused with normal saline, intramuscularly injected with CsA; The rabbits of LPS and CsA co-treatment group (LPS+CsA) were treated with LPS by intrauterine infusion and CsA by intramuscular injection. After 3 hours of treatment, collect uterine body tissue, that subsequently rapidly frozen by liquid nitrogen and then stored at -80℃.
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| Contributor(s) |
Xu L, Zeng Y, Chen M, Zeng Y, Zhang Y, Zhou G, Zeng C, Song T, Zhicai Z, Zhang M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 12, 2020 |
| Last update date |
Jun 15, 2020 |
| Contact name |
Ling xu |
| E-mail(s) |
xuling1114684263@126.com
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| Organization name |
Sichuan Agricultural University
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| Department |
College of animal science and technology
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| Street address |
No. 211 Huimin road
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
611130 |
| Country |
China |
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| Platforms (1) |
| GPL18453 |
Illumina HiSeq 2000 (Oryctolagus cuniculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA639056 |
| SRA |
SRP267050 |
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