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Series GSE152407 Query DataSets for GSE152407
Status Public on Jun 28, 2021
Title Single-cell RNA-Seq reveals a highly coordinated transcriptional program in mouse oocytes during follicular formation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To delineate the developmental trajectory of oocyte during the process, we performed RNA-seq on single germ cells from newborn (P0.5) ovaries and identified a series of genes that may function between stage transitions. The significant decrease of meiosis-related genes and the dramatic increase of oocyte-specific genes marked the transition of germ cell to a functional oocyte. Our data highlighted the complexity of transcriptional control and depicted the regulatons and their regulatory networks in oocyte development. Besides that, our data first characterized patterns of alternative splicing events and linked isform usage differences to cellular stage transitions of folliculogenesis. In summary, this study reconstructed molecular cascades in oocytes and revealed the orchestration of transcription-splicing coupling regulation on follicle formation.
Overall design Total 146 single cells from newborn mice ovary were performed.
Contributor(s) Li J, Shu W, Hu Z, He Y, Chen Q
Citation(s) 34174788
Submission date Jun 12, 2020
Last update date Jul 21, 2021
Contact name Wenjie Shu
Organization name Beijing Institute of Radiation Medicine
Street address Taiping Road 27
City Beijing
ZIP/Postal code 100850
Country China
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (146)
GSM4614641 ovarian cells_S001
GSM4614642 ovarian cells_S002
GSM4614643 ovarian cells_S003
BioProject PRJNA639176
SRA SRP267138

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Supplementary file Size Download File type/resource
GSE152407_RAW.tar 81.5 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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