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Series GSE15247 Query DataSets for GSE15247
Status Public on Aug 31, 2009
Title Shg1p associated RNAs
Organism Saccharomyces cerevisiae
Experiment type Other
Summary RNA co-immunopurification with TAP-tagged Shg1p (a component of the Set1 histone methyltransferase complex, SET1C) from Saccharomyces cerevisiae. An untagged (BY4741) served as a control (Gerber et al. 2004). Cells were grown to mid-log phase in rich media and harvested by centrifugation. TAP-tagged Shg1 was affinity-purified from cell-free extracts with IgG sepharose and eluted with TEV protease. RNA was isolated from extract (=input) and from the purified protein sample with the RNAeasy Kit (Qiagen). RNAs were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast oligo microarrays over night at 42 degrees in formamide-based hybridization buffer. For the method see experimental procedure for RNA IP (Gerber/Dichtl).
 
Overall design An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
 
Contributor(s) Gerber A
Citation(s)
  • Halbach A, Zhang H, Wengi A, Jablonska Z et al. Cotranslational assembly of the yeast SET1C histone methyltransferase complex. EMBO J 2009 Oct 7;28(19):2959-70. PMID: 19713935
Submission date Mar 16, 2009
Last update date Mar 21, 2012
Contact name Andre Gerber
E-mail(s) andre.gerber@pharma.ethz.ch
Phone +41 44 6337378
Fax +41 44 6337364
Organization name Swiss Federal Institute of Technology
Department Institute of Pharmaceutical Science
Street address Wolfgang Pauli Str. 10, HCI H396
City ETH Hoenggerberg, Zuerich
ZIP/Postal code ZH
Country Switzerland
 
Platforms (1)
GPL8306 SMD Print_1381 Saccharomyces cerevisiae
Samples (8)
GSM380861 Mock IP (3)
GSM380862 Shg1 IP (1)
GSM380863 Mock IP (2)
Relations
BioProject PRJNA116537

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15247_RAW.tar 1.0 Mb (http)(custom) TAR
Processed data included within Sample table

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