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Series GSE15247 Query DataSets for GSE15247
Status Public on Aug 31, 2009
Title Shg1p associated RNAs
Organism Saccharomyces cerevisiae
Experiment type Other
Summary RNA co-immunopurification with TAP-tagged Shg1p (a component of the Set1 histone methyltransferase complex, SET1C) from Saccharomyces cerevisiae. An untagged (BY4741) served as a control (Gerber et al. 2004). Cells were grown to mid-log phase in rich media and harvested by centrifugation. TAP-tagged Shg1 was affinity-purified from cell-free extracts with IgG sepharose and eluted with TEV protease. RNA was isolated from extract (=input) and from the purified protein sample with the RNAeasy Kit (Qiagen). RNAs were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast oligo microarrays over night at 42 degrees in formamide-based hybridization buffer. For the method see experimental procedure for RNA IP (Gerber/Dichtl).
 
Overall design An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.
 
Contributor(s) Gerber A
Citation(s) 19713935
Submission date Mar 16, 2009
Last update date Mar 21, 2012
Contact name Andre Gerber
E-mail(s) andre.gerber@pharma.ethz.ch
Phone +41 44 6337378
Fax +41 44 6337364
Organization name Swiss Federal Institute of Technology
Department Institute of Pharmaceutical Science
Street address Wolfgang Pauli Str. 10, HCI H396
City ETH Hoenggerberg, Zuerich
ZIP/Postal code ZH
Country Switzerland
 
Platforms (1)
GPL8306 SMD Print_1381 Saccharomyces cerevisiae
Samples (8)
GSM380861 Mock IP (3)
GSM380862 Shg1 IP (1)
GSM380863 Mock IP (2)
Relations
BioProject PRJNA116537

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15247_RAW.tar 1.0 Mb (http)(custom) TAR
Processed data included within Sample table

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