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Status |
Public on Jun 16, 2021 |
Title |
Effect of FGF10 on human Embryonic Stem Cells (hESC) and induced Pluripotent Stem Cells (hiPSC) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We wish to assess the transcritpomic regulation of FGF10 on human Embryonic Stem Cells (hESC) and human induced Pluripotent Stem Cells (hiPSC) that have been induced to express the earliest lung progenitor genetic marker, NKX2.1. Shortly after NKX2.1 was expressed in these cells, they were reseeded and FGF10 was added to the culture medium (experimental conditions) for either 12 or 24 hrs. Control samples had no FGF10 added. After the desired time, cells were harvested and stored in Trizol lysis buffer. RNA was extracted using a miRNeasy mini kit, and used for gene array analysis. Since this is a pilot experiment, we only have n=1 for experimental samples and n=1 for control samples for each condition.
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Overall design |
8 groups, each with 1 biological replicates
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Contributor(s) |
Jones M, Wilhelm J |
Citation missing |
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Submission date |
Jun 16, 2020 |
Last update date |
Jun 19, 2021 |
Contact name |
Jochen Wilhelm |
Organization name |
Justus-Liebig University Giessen
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Department |
Institute for Lung Health
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Lab |
ILH Genomics
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Street address |
Aulweg 132
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City |
Giessen |
State/province |
Hessen |
ZIP/Postal code |
35392 |
Country |
Germany |
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Platforms (1) |
GPL20844 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Feature Number Version] |
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Samples (8)
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Relations |
BioProject |
PRJNA639817 |