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Series GSE152597 Query DataSets for GSE152597
Status Public on Jun 16, 2021
Title Effect of FGF10 on human Embryonic Stem Cells (hESC) and induced Pluripotent Stem Cells (hiPSC)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We wish to assess the transcritpomic regulation of FGF10 on human Embryonic Stem Cells (hESC) and human induced Pluripotent Stem Cells (hiPSC) that have been induced to express the earliest lung progenitor genetic marker, NKX2.1. Shortly after NKX2.1 was expressed in these cells, they were reseeded and FGF10 was added to the culture medium (experimental conditions) for either 12 or 24 hrs. Control samples had no FGF10 added. After the desired time, cells were harvested and stored in Trizol lysis buffer. RNA was extracted using a miRNeasy mini kit, and used for gene array analysis. Since this is a pilot experiment, we only have n=1 for experimental samples and n=1 for control samples for each condition.
 
Overall design 8 groups, each with 1 biological replicates
 
Contributor(s) Jones M, Wilhelm J
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Submission date Jun 16, 2020
Last update date Jun 19, 2021
Contact name Jochen Wilhelm
Organization name Justus-Liebig University Giessen
Department Institute for Lung Health
Lab ILH Genomics
Street address Aulweg 132
City Giessen
State/province Hessen
ZIP/Postal code 35392
Country Germany
 
Platforms (1)
GPL20844 Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Feature Number Version]
Samples (8)
GSM4618625 hiPSC_cont_24h_rep1
GSM4618626 hESC_FGF10_12h_rep1
GSM4618627 hESC_cont_12h_rep1
Relations
BioProject PRJNA639817

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE152597_RAW.tar 63.2 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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