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| Status |
Public on Mar 31, 2022 |
| Title |
RNAseq of GL261 tumor endothelial cells from wild type and Eltd1-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Eltd1 is widely expressed in endothelial cells. We have reported that the expression of Eltd1 is highly up-regulated in glioma vessels. Other studies have shown that Eltd1 is up-regulated in vessels in other types of tumors, and has been suggested as a vascular target for anti-angiogenesis therapy. However, the role of Eltd1 in vessels is largely unknown. We isolated endothelial cells from healthy brain tissue and GL261 tumor tissue, and analyzed the role of Eltd1 in tumor angiogenesis by analyzing the endothelial gene profile from wild type and Eltd1-/- mice.
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| Overall design |
We injected GL261 intracranially to C57BL/6 wild type and Eltd1-/- mice. Tumors were dissected under a stereological microscope. To isolate endothelial cell mRNA from heathy brain tissue and tumor tissue, we took advantage of the mouse that expressing a fusion eGFP-L10a ribosomal protein under control of the VE-cadherin promoter, and crossed it with the Eltd1-/- mouse. Using translating ribosome affinity purification (TRAP) strategy, endothelial specific mRNA was isolated and were further prepared for RNAseq analysis.
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| Contributor(s) |
Huang H, Georganaki M, Dimberg A |
| Citation(s) |
34347079 |
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| Submission date |
Jun 16, 2020 |
| Last update date |
Apr 01, 2022 |
| Contact name |
L. H. |
| Organization name |
Uppsala University
|
| Street address |
Dag Hammarskjölds Väg 20
|
| City |
Uppsala |
| ZIP/Postal code |
751 85 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (11)
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| GSM4622575 |
Sample 1_Endothelial cells from tumor grown on Eltd1-/- mice |
| GSM4622576 |
Sample 2_Endothelial cells from tumor grown on Eltd1-/- mice |
| GSM4622577 |
Sample 3_Endothelial cells from tumor grown on wild type mice |
| GSM4622578 |
Sample 4_Endothelial cells from tumor grown on wild type mice |
| GSM4622579 |
Sample 5_Endothelial cells from tumor grown on wild type mice |
| GSM4622580 |
Sample 6_Endothelial cells from wild type mice brain |
| GSM4622581 |
Sample 7_Endothelial cells from wild type mice brain |
| GSM4622582 |
Sample 8_Endothelial cells from wild type mice brain |
| GSM4622583 |
Sample 9_Endothelial cells from Eltd1-/- mice brain |
| GSM4622584 |
Sample 10_Endothelial cells from Eltd1-/- mice brain |
| GSM4622585 |
Sample 11_Endothelial cells from Eltd1-/- mice brain |
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| Relations |
| BioProject |
PRJNA639870 |
| SRA |
SRP267581 |
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