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| Status |
Public on Jun 18, 2020 |
| Title |
Seminal Plasma-Derived Extracellular-Vesicle Fractions from HIV-Infected Men Exhibit Unique MicroRNA Signatures and Induce a Proinflammatory Response in Cells Isolated from the Female Reproductive Tract |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The continuing spread of HIV/AIDS is predominantly fueled by sexual exposure to HIV-contaminated semen. Seminal plasma (SP), the liquid portion of semen, harbors a variety of factors that may favor HIV transmission by facilitating viral entry into host cells, eliciting the production of pro-inflammatory cytokines, and enhancing the translocation of HIV across the genital epithelium. One important and abundant class of factors in SP is extracellular vesicles (EVs), which in general are important intercellular signal transducers. Although numerous studies have characterized blood plasma-derived EVs from both uninfected and HIV-infected individuals, little is known about the properties of EVs from semen of HIV-infected individuals. We report here that fractionated SP enriched for EVs from HIV-infected men induce potent transcriptional responses in epithelial and stromal cells that interface with luminal contents of the female reproductive tract. Compared to semen EV fractions from uninfected individuals, those from acutely-infected individuals induced a more pro-inflammatory signature. This was not associated with any observable differences in the surface phenotypes of the vesicles. However, miRNA expression profiling analysis revealed that EV fractions from infected individuals exhibit a broader and more diverse profile. Taken together, our data suggest that SP EVs from HIV-infected individuals exhibit unique miRNA signatures and exert potent pro-inflammatory transcriptional changes in cells of the female reproductive tract, which may facilitate HIV transmission.
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| Overall design |
The seminal plasma-derived microvesicle fractions (from 15 acutely-infected individuals and from 15 uninfected individuals) were assessed for their effects on endometrial stromal fibroblasts (n=3 donors) and endometrial epithelial cells (n=3 donors) by RNAseq.
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| Contributor(s) |
Marques de Menezes EG, Jang K, George AF, Nyegaard M, Neidleman J, Inglis HC, Danesh A, Deng X, Afshari A, Kim YH, Billaud J, Marson K, Pilcher CD, Pillai SK, Norris PJ, Roan NR |
| Citation(s) |
32434889 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R21 AI122821 |
Characterization of Exosomes From Semen of Uninfected and HIV-Infected Men |
University of California San Francisco |
Nadia R Roan |
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| Submission date |
Jun 17, 2020 |
| Last update date |
Sep 17, 2020 |
| Contact name |
Nadia Roan |
| E-mail(s) |
roann@urology.ucsf.edu
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| Organization name |
University of California at San Francisco
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| Street address |
1650 Owens Street
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| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (30)
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| GSM4624652 |
eEC_Mock_1 (Seminal Plasma-Derived Extracellular-Vesicle control) |
| GSM4624653 |
eEC_Mock_2 (Seminal Plasma-Derived Extracellular-Vesicle control) |
| GSM4624654 |
eEC_Mock_3 (Seminal Plasma-Derived Extracellular-Vesicle control) |
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| Relations |
| BioProject |
PRJNA640173 |
| SRA |
SRP267740 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE152714_Normalized_counts_eEC.xlsx |
4.5 Mb |
(ftp)(http) |
XLSX |
| GSE152714_Normalized_counts_eSF.xlsx |
4.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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