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| Status |
Public on Dec 22, 2020 |
| Title |
The regulatory genome of the malaria vector Anopheles gambiae: integrating chromatin accessibility and gene expression |
| Organism |
Anopheles gambiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Anopheles gambiae mosquitoes are primary human malaria vectors, but we know very little about their mechanisms of transcriptional regulation. We profiled chromatin accessibility by ATAC-seq in laboratory-reared An. gambiae mosquitoes experimentally infected with the human malaria parasite Plasmodium falciparum. By integrating ATAC-seq, RNA-seq and ChIP-seq data we showed a positive correlation between accessibility at promoters and introns, gene expression and active histone marks. By comparing expression and chromatin structure patterns in different tissues, we were able to infer cis-regulatory elements controlling tissue specific gene expression and to predict the in vivo binding sites of relevant transcription factors. The ATAC-seq assay also allowed the precise mapping of active regulatory regions, including novel transcription start sites and enhancers that annotate to mosquito immune-response genes. This study is important not only for advancing our understanding of mechanisms of transcriptional regulation in the mosquito vector of human malaria, but also the information we produced is of great potential for developing new mosquito-control and anti-malaria strategies.
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| |
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| Overall design |
Genome-wide profiling of chromatin accessibility (ATAC-seq) in mosquito tissues infected by P. falciparum coupled with transcriptome profiling (RNA-seq).
|
| Web link |
https://doi.org/10.1093/nargab/lqaa113
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| |
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| Contributor(s) |
Ruiz JL, Ranford-Cartwright LC, Gómez-Díaz E |
| Citation(s) |
33987532 |
| |
| Submission date |
Jun 21, 2020 |
| Last update date |
May 20, 2021 |
| Contact name |
Jose Luis Ruiz Rodriguez |
| Organization name |
IPBLN-CSIC
|
| Street address |
Avda. del Conocimiento 17. P. T. Ciencias de la Salud
|
| City |
Granada |
| State/province |
Granada |
| ZIP/Postal code |
18016 |
| Country |
Spain |
| |
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| Platforms (2) |
| GPL22033 |
Illumina NextSeq 500 (Anopheles gambiae) |
| GPL28724 |
Illumina HiSeq 4000 (Anopheles gambiae) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA640953 |
| SRA |
SRP268214 |
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