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Series GSE15342 Query DataSets for GSE15342
Status Public on Apr 03, 2009
Title Microarray analysis of gene expression in the skin of tick-naïve and tick-infested Holstein-Friesian and Brahman cattle
Platform organism Bos taurus
Sample organisms Bos taurus; Bos indicus
Experiment type Expression profiling by array
Summary This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus

Keywords: Disease state analysis
Overall design RNA was extracted from skin samples of tick-naïve cattle (animals with no previous R.microplus exposure) and tick-infested cattle after a period of successive, heavy infestations with R. microplus. Skin samples taken from tick-infested animals were taken at sites where tick larvae (approximately 24 h old) were attached to the skin sample. Skin samples were of 8 mm diameter and full skin thickness (approximately 10 mm). RNA samples from 12 animals (3 tick-naive Holstein-Friesian, 3 tick-naive Brahman, 3 tick-infested Holstein-Friesian and 3 tick-infested Brahman) were processed and hybridised to individual slides.
Contributor(s) Piper EK, Jonsson NN, Jackson LA, Lew AE, Gondro C
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Submission date Mar 23, 2009
Last update date Dec 17, 2012
Contact name Emily Kate Piper
Organization name University of Queensland
Department School of Veterinary Science
Street address Seddon Buildings (Bldg 82)
City Brisbane
ZIP/Postal code 4072
Country Australia
Platforms (1)
GPL2112 [Bovine] Affymetrix Bovine Genome Array
Samples (12)
GSM385316 Naïve Holstein 1
GSM385317 Naïve Holstein 2
GSM385318 Naïve Holstein 3
BioProject PRJNA116571

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15342_RAW.tar 40.1 Mb (http)(custom) TAR (of CEL) 2.8 Mb (ftp)(http) ZIP
Processed data included within Sample table

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