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| Status |
Public on Apr 03, 2009 |
| Title |
Microarray analysis of gene expression in the skin of tick-naïve and tick-infested Holstein-Friesian and Brahman cattle |
| Platform organism |
Bos taurus |
| Sample organisms |
Bos taurus; Bos indicus |
| Experiment type |
Expression profiling by array
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| Summary |
This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus
Keywords: Disease state analysis
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| Overall design |
RNA was extracted from skin samples of tick-naïve cattle (animals with no previous R.microplus exposure) and tick-infested cattle after a period of successive, heavy infestations with R. microplus. Skin samples taken from tick-infested animals were taken at sites where tick larvae (approximately 24 h old) were attached to the skin sample. Skin samples were of 8 mm diameter and full skin thickness (approximately 10 mm). RNA samples from 12 animals (3 tick-naive Holstein-Friesian, 3 tick-naive Brahman, 3 tick-infested Holstein-Friesian and 3 tick-infested Brahman) were processed and hybridised to individual slides.
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| Contributor(s) |
Piper EK, Jonsson NN, Jackson LA, Lew AE, Gondro C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 23, 2009 |
| Last update date |
Dec 17, 2012 |
| Contact name |
Emily Kate Piper |
| E-mail(s) |
e.piper@uq.edu.au
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| Organization name |
University of Queensland
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| Department |
School of Veterinary Science
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| Street address |
Seddon Buildings (Bldg 82)
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| City |
Brisbane |
| ZIP/Postal code |
4072 |
| Country |
Australia |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA116571 |
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