|
Status |
Public on Dec 08, 2020 |
Title |
Single cell ATAC-seq of neonatal heart regeneration |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
The adult mammalian heart has limited capacity for regeneration following injury, whereas the neonatal heart can readily regenerate within a short period after birth. Neonatal heart regeneration is orchestrated by multiple cell types intrinsic to the heart, as well as immune cells that infiltrate the heart after injury. To elucidate the transcriptional responses of the different cellular components of the mouse heart following injury, we performed single cell RNA-sequencing on neonatal hearts at various time points following myocardial infarction, and coupled the results with bulk tissue RNA-sequencing data collected at the same time points. Concomitant single cell ATAC-sequencing exposed underlying dynamics of open chromatin landscapes and regenerative gene regulatory networks of diverse cardiac cell types, and revealed previously unknown mediators of cardiomyocyte proliferation, angiogenesis and fibroblast activation. Together, our data provide a transcriptional basis for neonatal heart regeneration at single cell resolution and suggest new strategies for enhancing cardiac function in response to injury.
|
|
|
Overall design |
We performed myocardial infarction (MI) or sham surgeries to mouse hearts at postnatal day 1 or day 8. At 3d post P1 and P8 surgery, ventricle samples below the surgery plane were collected for single cell ATAC-Seq.
|
|
|
Contributor(s) |
Wang Z, Tan W, Olson E |
Citation(s) |
33296652 |
|
Submission date |
Jun 29, 2020 |
Last update date |
Jan 04, 2021 |
Contact name |
Zhaoning Wang |
E-mail(s) |
zhw063@health.ucsd.edu
|
Organization name |
UC San Diego
|
Department |
Cellular and Molecular Medicine
|
Lab |
Bing Ren Lab
|
Street address |
9500 Gilman Drive
|
City |
La Jolla |
State/province |
California |
ZIP/Postal code |
92093 |
Country |
USA |
|
|
Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
Samples (4)
|
|
This SubSeries is part of SuperSeries: |
GSE153481 |
Cell-type-specific gene regulatory networks underlying murine neonatal heart regeneration at single-cell resolution |
|
Relations |
BioProject |
PRJNA642813 |
SRA |
SRP269201 |