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Series GSE153514 Query DataSets for GSE153514
Status Public on Jun 30, 2020
Title Global Gene Expression Characterization of Circulating Tumor Cells in Metastatic Castration-Resistant Prostate Cancer Patients
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: Current therapeutic options in the course of metastatic castration-resistant prostate cancers (mCRPC) reinforce the need for reliable tools to characterize the tumor in a dynamic way. Circulating tumor cells (CTCs) have emerged as a viable solution to the problem, whereby patients with a variety of solid tumors, including PC, often do not have recent tumor tissue available for analysis. The biomarker characterization in CTCs could provide insights into the current state of the disease and an overall picture of the intra-tumor heterogeneity. Methods: in the present study, we applied a global gene expression characterization of the CTC population from mCRPC (n = 9), with the goal to better understand the biology of these cells and identify the relevant molecules favoring this tumor progression. Results: This analysis allowed the identification of 50 genes specifically expressed in CTCs from patients. Six of these markers (HOXB13, QKI, MAOA, MOSPD1, SDK1, and FGD4), were validated in a cohort of 28 mCRPC, showing clinical interest for the management of these patients. Of note, the activity of this CTC signature was related to the regulation of MYC, a gene strongly implicated in the biology of mCRPC. Conclusions: Overall, our results represent new evidence on the great value of CTCs as a non-invasive biopsy to characterize PC.
 
Overall design Briefly, the CTCs were immunoisolated from 7.5 mL of peripheral blood from mCRPC patients (n = 9) at baseline. For that, we used magnetic beads coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCAM). RNA from isolated CTCs was purified using a kit specifically designed for samples with low cellularity. In parallel, the same protocol was applied to blood samples from healthy donors (n = 6) to establish the baseline of background from unspecific non-CTC immunoisolation. A total RNA extraction, complete Whole Transcriptome Amplification, and gene expression array were performed on samples from the 9 patients and controls.
 
Contributor(s) León-Mateos L, Abalo A, Casas H, Anido U, Rapado-González Ó, Vieito M, Suárez-Cunqueiro M, Gómez-Tato A, Abal M, López-López R, Muinelo-Romay L
Citation(s)
  • León-Mateos L, Abalo A, Casas H, Anido U et al. Global Gene Expression Characterization of Circulating Tumor Cells in Metastasic Castration-Resistant Prostate Cancer Patients. J Clin Med 2020 Jul 1;9(7). PMID: 32630240
Submission date Jun 29, 2020
Last update date Aug 24, 2020
Contact name Laura Muinelo Romay
E-mail(s) lmuirom@gmail.com
Phone 654399833
Organization name IDIS
Department Liquid Biopsy Analysis Unit
Lab Laura Muinelo Romay
Street address Rua Magdalena 11, 3J
City SANTIAGO DE COMPOSTELA
State/province A Coruña
ZIP/Postal code 15895
Country Spain
 
Platforms (1)
GPL10332 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version)
Samples (24)
GSM4646271 Patient 2 basal
GSM4646272 Patient 2 progresion
GSM4646273 Patient 5 basal
Relations
BioProject PRJNA642874

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE153514_RAW.tar 226.7 Mb (http)(custom) TAR (of TXT)
GSE153514_normalized_by_probe_id.txt.gz 3.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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