|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Jun 30, 2020 |
Title |
Global Gene Expression Characterization of Circulating Tumor Cells in Metastatic Castration-Resistant Prostate Cancer Patients |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
Background: Current therapeutic options in the course of metastatic castration-resistant prostate cancers (mCRPC) reinforce the need for reliable tools to characterize the tumor in a dynamic way. Circulating tumor cells (CTCs) have emerged as a viable solution to the problem, whereby patients with a variety of solid tumors, including PC, often do not have recent tumor tissue available for analysis. The biomarker characterization in CTCs could provide insights into the current state of the disease and an overall picture of the intra-tumor heterogeneity. Methods: in the present study, we applied a global gene expression characterization of the CTC population from mCRPC (n = 9), with the goal to better understand the biology of these cells and identify the relevant molecules favoring this tumor progression. Results: This analysis allowed the identification of 50 genes specifically expressed in CTCs from patients. Six of these markers (HOXB13, QKI, MAOA, MOSPD1, SDK1, and FGD4), were validated in a cohort of 28 mCRPC, showing clinical interest for the management of these patients. Of note, the activity of this CTC signature was related to the regulation of MYC, a gene strongly implicated in the biology of mCRPC. Conclusions: Overall, our results represent new evidence on the great value of CTCs as a non-invasive biopsy to characterize PC.
|
|
|
Overall design |
Briefly, the CTCs were immunoisolated from 7.5 mL of peripheral blood from mCRPC patients (n = 9) at baseline. For that, we used magnetic beads coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCAM). RNA from isolated CTCs was purified using a kit specifically designed for samples with low cellularity. In parallel, the same protocol was applied to blood samples from healthy donors (n = 6) to establish the baseline of background from unspecific non-CTC immunoisolation. A total RNA extraction, complete Whole Transcriptome Amplification, and gene expression array were performed on samples from the 9 patients and controls.
|
|
|
Contributor(s) |
León-Mateos L, Abalo A, Casas H, Anido U, Rapado-González Ó, Vieito M, Suárez-Cunqueiro M, Gómez-Tato A, Abal M, López-López R, Muinelo-Romay L |
Citation(s) |
- León-Mateos L, Abalo A, Casas H, Anido U et al. Global Gene Expression Characterization of Circulating Tumor Cells in Metastasic Castration-Resistant Prostate Cancer Patients. J Clin Med 2020 Jul 1;9(7). PMID: 32630240
|
|
Submission date |
Jun 29, 2020 |
Last update date |
Aug 24, 2020 |
Contact name |
Laura Muinelo Romay |
E-mail(s) |
lmuirom@gmail.com
|
Phone |
654399833
|
Organization name |
IDIS
|
Department |
Liquid Biopsy Analysis Unit
|
Lab |
Laura Muinelo Romay
|
Street address |
Rua Magdalena 11, 3J
|
City |
SANTIAGO DE COMPOSTELA |
State/province |
A Coruña |
ZIP/Postal code |
15895 |
Country |
Spain |
|
|
Platforms (1) |
GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
|
Samples (24)
|
|
Relations |
BioProject |
PRJNA642874 |
Supplementary file |
Size |
Download |
File type/resource |
GSE153514_RAW.tar |
226.7 Mb |
(http)(custom) |
TAR (of TXT) |
GSE153514_normalized_by_probe_id.txt.gz |
3.4 Mb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
Processed data provided as supplementary file |
Processed data are available on Series record |
|
|
|
|
|