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Series GSE153517 Query DataSets for GSE153517
Status Public on Jun 30, 2020
Title Fibroblasts in Nodular Sclerosing Classical Hodgkin Lymphoma Are Defined by a Specific Phenotype and Protect Tumor Cells From Brentuximab-Vedotin Induced Injury
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Several studies have described a crosstalk between the tumour cells of cHL, the Hodgkin- and Reed-Sternberg (HRS) cells, and cancer-associated fibroblasts (CAF). However, to date a deep molecular characterization of these fibroblasts is lacking. Aim of the present study therefore was a comprehensive characterization of these fibroblasts.
 
Overall design Gene expression of fibroblasts isolated from primary lymph node suspensions (LA = lymphoadenitis, n=7, cHL_MC = classical Hodgkin lymphoma mixed cellularity subtype, n=5; cHL_NS = classical Hodgkin lymphoma nodular sclerosing subtype, n=8; Fib = primary fibroblasts culture at passage 4-6 in vitro, KB = arbitrary sample ID prefix, FST = arbitrary sample ID prefix). As previously shown for other types of cancer-associated fibroblasts, treatment by luteolin could reverse this fibroblast phenotype. Working concentration of 30 µM luteolin was added to sample KB33 and harvested 48 h post treatment for comparative analysis with Fib_cHL_NS samples. Three biological replicates were applied. The cHL specific adherence-based interaction of both cell fractions revealed an initiation of inflammatory key regulators. The classical Hodgkin lymphoma cell line L-428 and adherent activated fibroblasts cells from primary nodular sclerosis cHL were used. Floating cell fractions were removed by repeated washing. Adherent interacting coculture layer was harvested for comparative gene expression analysis to mock coculture. Mock coculture was set up separated in equivalent ratios and pooled before RNA analysis. Three biological replicates were applied.
 
Contributor(s) Bankov K, Döring C
Citation(s) 31671543
Submission date Jun 29, 2020
Last update date Jul 02, 2020
Contact name Claudia Döring
E-mail(s) C.Doering@em.uni-frankfurt.de
Organization name Johann Wolfgang-Goethe University Frankfurt a.M.
Department Medical School, Institite of Pathology
Street address Theodor Stern Kai 7
City Frankfurt
ZIP/Postal code 60590
Country Germany
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (34)
GSM4646305 Fibroblast culture derived from primary lymph node KB34_
GSM4646306 Fibroblast culture derived from primary lymph node KB35_
GSM4646307 Fibroblast culture derived from primary lymph node KB36_
Relations
BioProject PRJNA642881

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Supplementary file Size Download File type/resource
GSE153517_RAW.tar 129.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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