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| Status |
Public on Mar 23, 2009 |
| Title |
Genome-wide profiles of STAT1 DNA association using chromatin immunoprecipitation and massively parallel sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We developed a method, ChIP-sequencing (ChIP-seq), combining chromatin immunoprecipitation (ChIP) and massively parallel sequencing to identify mammalian DNA sequences bound by transcription factors in vivo. We used ChIP-seq to map STAT1 targets in interferon stimulated and unstimulated human HeLa S3 cells, and compared the method's performance to ChIP-PCR and to ChIP-chip for four chromosomes. By ChIP-seq, using 15.1 and 12.9 million uniquely mapped sequence reads, and an estimated false discovery rate of less than 0.001, we identified 41,582 and 11,004 putative STAT1-binding regions in stimulated and unstimulated cells, respectively. Of the 34 loci known to contain STAT1 interferon-responsive binding sites, ChIP-seq found 24 (71%). ChIP-seq targets were enriched in sequences similar to known STAT1 binding motifs. Comparisons with two ChIP-PCR data sets suggested that ChIP-seq sensitivity was between 70% and 92% and specificity was at least 95%.
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| Overall design |
Use ChIP-seq to map STAT1 targets in interferon-gamma (IFN-gamma)-stimulated and unstimulated human HeLa S3 cells, and compared the method's performance to ChIP-PCR and to ChIP-chip for four chromosomes
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| Contributor(s) |
Robertson G, Hirst M, Bainbridge M, Bilenky M, Zhao Y, Zeng T, Euskirchen G, Bernier B, Varhol R, Delaney A, Thiessen N, Griffith OL, He A, Marra M, Snyder M, Jones S |
| Citation(s) |
17558387 |
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| Submission date |
Mar 23, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Eric Chuah |
| E-mail(s) |
echuah@bcgsc.ca
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| Phone |
604-707-5900 3231
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| Organization name |
BC Genome Sciences Centre
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| Street address |
570 West 7th Ave
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| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4S6 |
| Country |
Canada |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (13)
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| Relations |
| SRA |
SRP000661 |
| BioProject |
PRJNA115767 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15353_STAT1_hg18_IFNg_ht11.peaks.txt |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE15353_STAT1_hg18_IFNg_ht11.wig |
96.2 Mb |
(ftp)(http) |
WIG |
| GSE15353_STAT1_hg18_Unstimulated_ht11.peaks.txt |
342.6 Kb |
(ftp)(http) |
TXT |
| GSE15353_STAT1_hg18_Unstimulated_ht11.wig |
21.6 Mb |
(ftp)(http) |
WIG |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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