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Status |
Public on Jan 21, 2021 |
Title |
Glycolytic metabolism of pathogenic T cells enables early detection of GvHD by 13C-MRI. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Graft-versus-host disease (GvHD) is a prominent barrier to allogeneic hematopoietic stem cell transplantation (HSCT). Definitive diagnosis of GvHD is invasive and biopsies of involved tissues pose a high risk of bleeding and infection. Our previous studies in a chronic GvHD mouse model demonstrated that alloreactive CD4+ T cells are distributed to target organs ahead of overt symptoms, meanwhile CD4+ T cell activation is tied to increased glycolysis. We aimed to compare the gene expression of allogeneic (mismatched) naive (Tn) and effector memory (Tem) CD4+ T cell subsets early post transplant. Specifically, we hypothesized that allogeneic CD4 effector memory T cells would show upregulated expression of genes related to glycolysis. We found that almost all enzymes of glycolysis were upregulated in effector memory CD4 T cells compared to naive cells, including transporters for glucose. In contrast, enzymes of the tricarboxylic acid cycle were not uniformly elevated.
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Overall design |
FACS-purified CD45+ CD4+ cells were isolated from syngeneic and allogeneic transplanted mice (n= 3 each) in a model of chronic GvHD on day 14 from the liver. After sorting, cells were counted, tested for viability and a target of 6000 cells was loaded onto the 10X system. The cells were processed according to the instructions for the 5' sequencing kit.
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Contributor(s) |
Buxbaum NP, Kelly M, Assmann JC |
Citation(s) |
- Assmann JC, Farthing DE, Saito K, Maglakelidze N et al. Glycolytic metabolism of pathogenic T cells enables early detection of GVHD by 13C-MRI. Blood 2021 Jan 7;137(1):126-137. PMID: 32785680
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Submission date |
Jun 30, 2020 |
Last update date |
Jan 21, 2021 |
Contact name |
Nataliya Prokopenko Buxbaum |
E-mail(s) |
nbuxbaum@mail.nih.gov
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Phone |
2407606157
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Organization name |
NCI, NIH
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Department |
ETIB
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Street address |
10 Center Drive, CRC, 3-3288
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City |
Bethesda |
State/province |
Maryland |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
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Samples (6)
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Relations |
BioProject |
PRJNA643283 |
SRA |
SRP269434 |