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Series GSE153949 Query DataSets for GSE153949
Status Public on Oct 27, 2020
Title An unorthodox flagellin paralog integrates cell cycle and flagellar assembly cues for flagellin translation (Tn-seq)
Organism Caulobacter vibrioides NA1000
Experiment type Other
Summary How cellular checkpoints couple the orderly assembly of macromolecular machines with cell cycle progression is poorly understood. The alpha-proteobacterium Caulobacter crescentus assembles a single polar flagellum during each cell cycle. We discovered that expression of multiple flagellin transcripts is licensed by a translational checkpoint responsive to a dual input signal: a secretion-competent hook-basal-body (HBB) structure and a surge in the FlaF secretion chaperone during cytokinesis, instructed by the cell cycle circuitry. We find that the unorthodox FljJ flagellin, one of six flagellin paralogs, acts as checkpoint linchpin, binding both FlaF and the FlbT translational regulator. FljJ recruits FlbT to inhibit translation at the 5’ untranslated region in other flagellin transcripts before HBB assembly. Once FlaF is synthesized and stabilized, it directs FljJ secretion through the HBB, thereby separating FlbT from its co-activator and relieving translational inhibition. The FlbT/FlaF pair is wide-spread and functional properties are conserved in alpha-proteobacteria, including pathogens.
 
Overall design Genome-wide transposon mutagenesis coupled with deep-sequencing (Tn-Seq) of Caulobacter crescentus NA1000 (WT) and ΔtipF pSA607 mutant.
 
Contributor(s) Ardissone S, Kint N, Petrignani B, Panis G, Viollier PH
Citation(s) 33113346
Submission date Jul 07, 2020
Last update date May 01, 2023
Contact name Patrick H. Viollier
E-mail(s) Patrick.Viollier@unige.ch
Organization name University of Geneva, Faculty of Medicine / CMU
Department Dept. Microbiology and Molecular Medicine
Street address Rue Michel Servet 1
City Geneva 4
ZIP/Postal code 1211
Country Switzerland
 
Platforms (1)
GPL26188 Illumina NextSeq 500 (Caulobacter vibrioides NA1000)
Samples (2)
GSM4658992 Tn-Seq_Caulobacter_crescentus_NA1000(WT)
GSM4658993 Tn-Seq_Caulobacter_crescentus_NA1000(tipFpSA607)
This SubSeries is part of SuperSeries:
GSE153955 An unorthodox flagellin paralog integrates cell cycle and flagellar assembly cues for flagellin translation
Relations
BioProject PRJNA644598
SRA SRP270670

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Supplementary file Size Download File type/resource
GSE153949_tipFpSA607_vs_WT_Tn-Seq_analysis--Raw_counts_and_CDS_counts_TnPM_normalized.xlsx 3.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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