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| Status |
Public on May 06, 2022 |
| Title |
Response of aspen cell cultures fed piperonylic acid to methyl jasmonate. |
| Platform organism |
Populus sp. |
| Sample organism |
Populus tremuloides |
| Experiment type |
Expression profiling by array
|
| Summary |
To better understand the role of phenylpropanoid pathway perturbation in plant metabolism, Populus tremuloides cell cultures previously established from leaf mesophyll tissue were fed with piperonylic acid (PIP) alone, or in combination with methyl jasmonate (MJ_PIP) approximately 5 days after transfer to fresh medium. Methyl jasmonate is an elicitor that activates a suite of defense responses including phenylpropanoid metabolism, while PIP acts as an inhibitor of cinnamate-4-hydroxylase (C4H). Sample tissues were contemporary with those used to generate Series GSE16773.
Samples were harvested 48 hrs after initiation of the experiment. Total RNA was extracted and gene expression measured using Affymetrix poplar genome microarrays.
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| Overall design |
Populus tremuloides cells suspended in liquid culture were fed with piperonylic acid dissolved in DMSO (PIP) either alone or in combination with methyl jasmonate in DMSO (MJ). Two replicates of total RNA were utilized for microarray analysis from a total of six or seen replicates produced per treatment condition.
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| Contributor(s) |
Payyavula R, Du L, Harding SA, Tsai C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 10, 2020 |
| Last update date |
May 07, 2022 |
| Contact name |
Chung-Jui Tsai |
| E-mail(s) |
cjtsai@uga.edu
|
| Organization name |
The University of Georgia
|
| Department |
Department of Genetics
|
| Street address |
120 Green Street
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
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| Platforms (1) |
| GPL4359 |
[Poplar] Affymetrix Poplar Genome Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA645444 |
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