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Series GSE154288 Query DataSets for GSE154288
Status Public on Feb 23, 2022
Title RNA Sequencing Facilitates Quantitative Analysis of Wild Type(MRL-mpj) and MRL-lpr (PBS or MSC treated) Hippocampoal Transcriptomes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Up to 75% of systematic lupus erythematosus (SLE) patients experience neuropsychiatric (NP) symptoms, called neuropsychiatric SLE (NPSLE), yet the underlying mechanisms remain elusive. Complement cascades mediate synaptic pruning by microglia during early postnatal brain development. The process in NPSLE remains unclear. Here, we show that complement-coordinated elimination of synaptic terminals participated in NPSLE in MRL/lpr mice, a lupus-prone murine model. We elucidated that lupus mice developed increased anxiety-like behaviors and persistent phagocytic microglia reactivation before overt peripheral lupus pathology. Microglial engulfment of synapses explained behavioral disorders. We further determined that neuronal Nr4a1 signaling was essential for attracting C1q synaptic deposition then apposition of phagocytic microglia, ensuing synaptic loss and neurological disease. Minocycline-deactivated microglia, antibody-blocked C1q, or neuronal Nr4a1 restore protected lupus mice from synapse loss and NP manifestations. Our findings revealed an active role of neurons in coordinating microglia-mediated synaptic loss and highlight neuronal Nr4a1 and C1q as critical components amenable to pharmacological intervention.
 
Overall design RNA sequencing in hippocampus tissues from WT(MRL-mpj) mice and MRL-lpr mice with or without MSC(mesenchymal stem cell) administration, in triplicate.
 
Contributor(s) Han X, Sun L
Citation(s) 35177587
Submission date Jul 13, 2020
Last update date Feb 24, 2022
Contact name Han Xiaojuan
E-mail(s) hxj_719@126.com
Phone 15951950715
Organization name Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School
Street address Zhongshan Road 321
City Nanjing
State/province Jiangsu
ZIP/Postal code 210008
Country China
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM4668496 Hippocampus WT1
GSM4668497 Hippocampus WT2
GSM4668498 Hippocampus WT3
Relations
BioProject PRJNA645810
SRA SRP271530

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154288_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
GSE154288_Raw_gene_counts_matrix.txt.gz 531.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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