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| Status |
Public on Dec 31, 2022 |
| Title |
Sea anemone mesentery muscle profiling [bulk RNA-seq] |
| Organism |
Nematostella vectensis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Isolated mesenteries from MHC::mCherry Adults were used to generate FACS-sorted RNA sequencing libraries in order to characterize gene use in Cnidarian smooth muscle. Transgenic mCherry expressing cells are compared to the non-expressing cells of the surrounding tissues, and up-regulated genes within the muscle cell population can be identified as important muscle-related gene products.
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| Overall design |
Adult mesenteries from eight Nematostella vectensis MHC::mCherry transgenic lines were isolated and dissociated into single cell suspensions, performed in duplicate. These were FACS-sorted into mCherry+ and mCherry- populations, used to generate four RNA sequencing libraries
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| |
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| Contributor(s) |
Cole AG, Technau U, Jahnel S |
| Citation(s) |
36130520, 36990990 |
| |
| Submission date |
Jul 15, 2020 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Alison G Cole |
| Organization name |
University of Vienna
|
| Department |
Neurosciences and Developmental Biology
|
| Street address |
Djerassiplatz 1
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL23802 |
Illumina HiSeq 2500 (Nematostella vectensis) |
|
| Samples (4)
|
| GSM4671900 |
Nematostella vectensis MHC::mCherry Adult mesentery_20953 |
| GSM4671901 |
Nematostella vectensis MHC::mCherry Adult mesentery_20954 |
| GSM4671902 |
Nematostella vectensis MHC::mCherry Adult mesentery_20955 |
| GSM4671903 |
Nematostella vectensis MHC::mCherry Adult mesentery_20956 |
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| This SubSeries is part of SuperSeries: |
| GSE154477 |
Transcriptomic profiling of sea anemone tissues |
|
| Relations |
| BioProject |
PRJNA646458 |
| SRA |
SRP272060 |
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