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Status |
Public on Jun 28, 2022 |
Title |
Identification of ALKBH5 targets in osteosarcoma by methyl RNA immunoprecipitation [MeRIP-seq] |
Organism |
Homo sapiens |
Experiment type |
Other
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Summary |
To identify potential target mRNAs that are demethylated by ALKBH5, meRIP-seq with a m6A antibody was conducted in 143B osteosarcoma cells transfected with scrambled or ALKBH5-siRNA.
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Overall design |
143B cells were grown in 150 mm tissue culture plates and transfected with with either scrambled-siRNA or ALKBH5-siRNAs (Sigma-Aldrich) for 48 hours.Total RNA / mRNA isolated from the 143B cells was fragmented and subjected to immunoprecipitation (IP) using rabbit anti-m6A antibody. After incubation and washing , samples were eluted using elution buffer containing m6A nucleoside . The resultant final product was used for RNA-seq.
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Contributor(s) |
Yadav P, Rao M, Chen Y, Lai Z |
Citation(s) |
35303054 |
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Submission date |
Jul 15, 2020 |
Last update date |
Jul 01, 2022 |
Contact name |
Yidong Chen |
E-mail(s) |
cheny8@uthscsa.edu
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Phone |
2105629163
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Organization name |
UT Health Science Center at San Antonio
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Department |
Population Health Sciences
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Street address |
8403 Floyd Curl Drive, MSC 7784
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City |
San Antonio |
State/province |
Texas |
ZIP/Postal code |
78229 |
Country |
USA |
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Platforms (1) |
GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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Samples (12)
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This SubSeries is part of SuperSeries: |
GSE154530 |
Gene regulation by m6A demethylase ALKBH5 in osteosarcoma |
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Relations |
BioProject |
PRJNA646541 |
SRA |
SRP272156 |