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| Status |
Public on Jun 28, 2022 |
| Title |
Identification of ALKBH5 targets in osteosarcoma by methyl RNA immunoprecipitation [MeRIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
To identify potential target mRNAs that are demethylated by ALKBH5, meRIP-seq with a m6A antibody was conducted in 143B osteosarcoma cells transfected with scrambled or ALKBH5-siRNA.
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| Overall design |
143B cells were grown in 150 mm tissue culture plates and transfected with with either scrambled-siRNA or ALKBH5-siRNAs (Sigma-Aldrich) for 48 hours.Total RNA / mRNA isolated from the 143B cells was fragmented and subjected to immunoprecipitation (IP) using rabbit anti-m6A antibody. After incubation and washing , samples were eluted using elution buffer containing m6A nucleoside . The resultant final product was used for RNA-seq.
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| Contributor(s) |
Yadav P, Rao M, Chen Y, Lai Z |
| Citation(s) |
35303054 |
| |
| Submission date |
Jul 15, 2020 |
| Last update date |
Jul 01, 2022 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
|
| Phone |
2105629163
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Population Health Sciences
|
| Street address |
8403 Floyd Curl Drive, MSC 7784
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE154530 |
Gene regulation by m6A demethylase ALKBH5 in osteosarcoma |
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| Relations |
| BioProject |
PRJNA646541 |
| SRA |
SRP272156 |
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