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Series GSE154569 Query DataSets for GSE154569
Status Public on Sep 16, 2020
Title CRISPR-Cas protein Cas3 controls virulence in the oral pathogen Porphyromonas gingivalis.
Organisms Porphyromonas gingivalis; Galleria mellonella
Experiment type Expression profiling by high throughput sequencing
Summary Our results show that compared to wild type, a deletion mutant of the cas3 gene, an essential nuclease part of the class 1 type I CRISPR-Cas system, increases the virulence of P gingivalis.
 
Overall design we performed targeted deletion of cas3 to understand type I CRISPR-Cas systems and because it was among the most up-regulated CRISPR-Cas genes in our metatranscriptomic study (30). Using dual transcriptomic evaluations, coupled with cell and infection assays, this study demonstrates that Cas3 controls P. gingivalis virulence upon intracellular infection, but seems to have no role in virulence when growing in the planktonic phase
 
Contributor(s) Solbiati J, Duran-Pinedo A, Rocha FG, Gibson FC, Frias-Lopez J
Citation(s) 32994292
Submission date Jul 16, 2020
Last update date Oct 06, 2020
Contact name Jose Solbiati
E-mail(s) josesolbiati@ufl.edu
Organization name University of Florida
Department Oral biology
Street address 1395 Center Drive
City Gainesville
State/province Florida
ZIP/Postal code 32610
Country USA
 
Platforms (2)
GPL24653 Illumina HiSeq 2500 (Porphyromonas gingivalis)
GPL25944 Illumina HiSeq 2500 (Galleria mellonella)
Samples (46)
GSM4674688 G.mellonela injected with WT Porphyromonas gingivalis Wax1
GSM4674689 G.mellonela injected with WT Porphyromonas gingivalis Wax2
GSM4674690 G.mellonela injected with WT Porphyromonas gingivalis Wax3
Relations
BioProject PRJNA646710
SRA SRP272245

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154569_Matrix_genes_counts_G.mellonella_KO.csv.gz 191.7 Kb (ftp)(http) CSV
GSE154569_Matrix_genes_counts_G.mellonella_WT.csv.gz 177.6 Kb (ftp)(http) CSV
GSE154569_Matrix_genes_counts_Pg_in_G.mellonella_KO.csv.gz 28.6 Kb (ftp)(http) CSV
GSE154569_Matrix_genes_counts_Pg_in_G.mellonella_WT.csv.gz 28.1 Kb (ftp)(http) CSV
GSE154569_Matrix_genes_counts_Pg_intracellular_in_THP-1_cells_KO.csv.gz 21.4 Kb (ftp)(http) CSV
GSE154569_Matrix_genes_counts_Pg_intracellular_in_THP-1_cells_WT.csv.gz 21.6 Kb (ftp)(http) CSV
GSE154569_Matrix_genes_counts_Pg_planktonic_growth_KO.csv.gz 22.6 Kb (ftp)(http) CSV
GSE154569_Matrix_genes_counts_Pg_planktonic_growth_WT.csv.gz 22.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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