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Status |
Public on Jul 21, 2020 |
Title |
Detection of RNAs binding to Vg1 RNP |
Organism |
Xenopus laevis |
Experiment type |
Other
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Summary |
We report the application of NGS for high-throughput profiling of transcripts assoicated with the ribonuleoprotein (RNP) formed on Vg1 mRNA in oocytes of Xenopus Laevis.
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Overall design |
Biriefly, Biotinylated-Vg1 RNA was microinjected into Xenopus oocyte to allow formation of RNP complex in vivo. Next, streptavidin beads were added to the oocyte extract to specificly pull down the biotin-Vg1 RNP. After extensive washes, biotin-Vg1 RNP were eluted off by incubating the beads with free biotin and the associted RNAs were extracted by phenol and precipitated by ethanol.For RNA sequencing strategy, a single RNA library was prepared and sequenced on an Illumina MiSeq nano 300 cycle reagent kit.
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Contributor(s) |
Yang C, Huber PW |
Citation missing |
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Submission date |
Jul 20, 2020 |
Last update date |
Jul 24, 2020 |
Contact name |
Chao Yang |
E-mail(s) |
cyang4@nd.edu
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Organization name |
University of Notre Dame
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Department |
Chemistry and Biochemistry
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Lab |
Paul Huber
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Street address |
437 Stepan Chemistry Hall
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City |
Notre Dame |
State/province |
IN |
ZIP/Postal code |
46556 |
Country |
USA |
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Platforms (1) |
GPL22426 |
Illumina MiSeq (Xenopus laevis) |
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Samples (1) |
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Relations |
BioProject |
PRJNA647395 |
SRA |
SRP272643 |