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Series GSE154766 Query DataSets for GSE154766
Status Public on Jul 21, 2020
Title Detection of RNAs binding to Vg1 RNP
Organism Xenopus laevis
Experiment type Other
Summary We report the application of NGS for high-throughput profiling of transcripts assoicated with the ribonuleoprotein (RNP) formed on Vg1 mRNA in oocytes of Xenopus Laevis.
 
Overall design Biriefly, Biotinylated-Vg1 RNA was microinjected into Xenopus oocyte to allow formation of RNP complex in vivo. Next, streptavidin beads were added to the oocyte extract to specificly pull down the biotin-Vg1 RNP. After extensive washes, biotin-Vg1 RNP were eluted off by incubating the beads with free biotin and the associted RNAs were extracted by phenol and precipitated by ethanol.For RNA sequencing strategy, a single RNA library was prepared and sequenced on an Illumina MiSeq nano 300 cycle reagent kit.
 
Contributor(s) Yang C, Huber PW
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Submission date Jul 20, 2020
Last update date Jul 24, 2020
Contact name Chao Yang
E-mail(s) cyang4@nd.edu
Organization name University of Notre Dame
Department Chemistry and Biochemistry
Lab Paul Huber
Street address 437 Stepan Chemistry Hall
City Notre Dame
State/province IN
ZIP/Postal code 46556
Country USA
 
Platforms (1)
GPL22426 Illumina MiSeq (Xenopus laevis)
Samples (1)
GSM4679353 Vg1 RNP
Relations
BioProject PRJNA647395
SRA SRP272643

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Supplementary file Size Download File type/resource
GSE154766_RAW.tar 1.0 Mb (http)(custom) TAR (of XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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