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GEO help: Mouse over screen elements for information. |
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Status |
Public on Jul 22, 2020 |
Title |
Limited proliferation capacity of aorta intima resident macrophages requires monocyte recruitment for atherosclerotic plaque progression |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Atherosclerosis is modulated by immune cells. Understanding the diversity and heterogeneity in the aorta in steady state, as well as during different stages of disease will help to determine mechanisms promoting disease progression. To characterize the immune cells, we performed single cell RNA sequencing of aortic CD45+ leukocytes from C57BL/6 (WT) on chow diet and early disease Ldlr-/- mice fed high fat diet (HFD) for 21-days. The single cell RNA-seq analyses revealed the heterogeneity of aortic macrophages in steady state and early atherosclerotic disease.
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Overall design |
Single cell RNA sequencing of aortic CD45+ cells from nascent (n=10, C57BL/6 8-weeks age) and early atherosclerotic Ldlr-/- mice (n=10, HFD 21-days), using Illumina HiSeq4000.
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Contributor(s) |
Williams JW, Zaitsev K, Kim K, Ivanov S, Saunders B, Schrank PR, Kim K, Elvington A, Kim SH, Tucker CG, Wohltmann M, Fife BT, Epelman S, Artyomov MN, Lavine KJ, Zinselmeyer BH, Choi J, Randolph GJ |
Citation(s) |
32895539 |
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Submission date |
Jul 21, 2020 |
Last update date |
Oct 23, 2020 |
Contact name |
Maxim Artyomov |
E-mail(s) |
martyomov@wustl.edu
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Organization name |
WASHINGTON UNIVERSITY IN ST LOUIS
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Department |
Pathology & Immunology
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Lab |
Artyomov lab
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Street address |
425 S Euclid Ave
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City |
St. Louis |
State/province |
Missouri |
ZIP/Postal code |
63110 |
Country |
USA |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (2) |
GSM4680682 |
Total CD45+ Leukocytes from C57BL/6 Aorta |
GSM4680683 |
Total CD45+ Leukocytes from Ldlr-/- Aorta |
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Relations |
BioProject |
PRJNA647813 |
SRA |
SRP273081 |
Supplementary file |
Size |
Download |
File type/resource |
GSE154817_RAW.tar |
59.7 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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