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Series GSE154817 Query DataSets for GSE154817
Status Public on Jul 22, 2020
Title Limited proliferation capacity of aorta intima resident macrophages requires monocyte recruitment for atherosclerotic plaque progression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Atherosclerosis is modulated by immune cells. Understanding the diversity and heterogeneity in the aorta in steady state, as well as during different stages of disease will help to determine mechanisms promoting disease progression. To characterize the immune cells, we performed single cell RNA sequencing of aortic CD45+ leukocytes from C57BL/6 (WT) on chow diet and early disease Ldlr-/- mice fed high fat diet (HFD) for 21-days. The single cell RNA-seq analyses revealed the heterogeneity of aortic macrophages in steady state and early atherosclerotic disease.
 
Overall design Single cell RNA sequencing of aortic CD45+ cells from nascent (n=10, C57BL/6 8-weeks age) and early atherosclerotic Ldlr-/- mice (n=10, HFD 21-days), using Illumina HiSeq4000.
 
Contributor(s) Williams JW, Zaitsev K, Kim K, Ivanov S, Saunders B, Schrank PR, Kim K, Elvington A, Kim SH, Tucker CG, Wohltmann M, Fife BT, Epelman S, Artyomov MN, Lavine KJ, Zinselmeyer BH, Choi J, Randolph GJ
Citation(s) 32895539
Submission date Jul 21, 2020
Last update date Oct 23, 2020
Contact name Maxim Artyomov
E-mail(s) martyomov@wustl.edu
Organization name WASHINGTON UNIVERSITY IN ST LOUIS
Department Pathology & Immunology
Lab Artyomov lab
Street address 425 S Euclid Ave
City St. Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (2)
GSM4680682 Total CD45+ Leukocytes from C57BL/6 Aorta
GSM4680683 Total CD45+ Leukocytes from Ldlr-/- Aorta
Relations
BioProject PRJNA647813
SRA SRP273081

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Supplementary file Size Download File type/resource
GSE154817_RAW.tar 59.7 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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