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Series GSE154896 Query DataSets for GSE154896
Status Public on Jul 21, 2021
Title Single-cell transcriptomic analysis reveals unique cell types and patterns of gene expression in the frog corneal epithelium.
Organism Xenopus laevis
Experiment type Expression profiling by high throughput sequencing
Summary Here, we use scRNA-seq to expand our understanding of the vertebrate corneal epithelium in an amphibian model, having undertaken a comprehensive characterization of cellular and transcriptional heterogeneity to identify novel corneal marker genes.
 
Overall design Adult Xenopus laevis were obtained from Nasco (Fort Atkinson, WI). Fertilized eggs were collected, and larvae were raised to Stage 49-51 based on developmental staging by Nieuwkoop and Faber (1956). The corneal epithelial tissue was dissected using very fine microscissors (Henry et al., 2019), taking care not to include any surrounding skin. Corneal tissue was dissociated and processed through the 10X pipeline, and we identified 5173 cells using Cellranger.
 
Contributor(s) Bangru S, Sonam S, Henry JJ
Citation(s) 36049533
Submission date Jul 22, 2020
Last update date Sep 07, 2023
Contact name Sushant Bangru
E-mail(s) bangru2@illinois.edu, sushantbangru@gmail.com, sb725@duke.edu
Phone +1-217-979-9320
Organization name University of Illinois, Urbana-Champaign
Department Biochemistry
Lab Dr. Auinash Kalsotra
Street address 600 S Mathews Avenue
City Urbana
State/province Illinois
ZIP/Postal code 61801
Country USA
 
Platforms (1)
GPL28901 Illumina NovaSeq 6000 (Xenopus laevis)
Samples (1)
GSM4681962 Cornea Stage 49-51
Relations
BioProject PRJNA647844
SRA SRP273066

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE154896_RAW.tar 95.8 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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