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| Status |
Public on Jul 23, 2020 |
| Title |
Host immune response against the emerging fungal pathogen Candida auris: transcriptional and functional insights |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Candida auris is amongst the most important emerging fungal pathogens, yet mechanistic insights in its immune recognition and control are lacking. Here, we integrate transcriptional and functional immune cell profiling to uncover innate anti-C. auris defense mechanisms. C. auris induces a specific transcriptome in human mononuclear cells, a stronger cytokine response compared to C. albicans, but a lower macrophage lysis capacity. C. auris-induced innate immune activation is mediated through recognition of C-type lectin receptors, mainly elicited by structurally unique C. auris mannoproteins. In in-vivo experimental models of disseminated candidiasis, C. auris was less virulent than C. albicans. Collectively, these results demonstrate that C. auris is a strong inducer of innate host defense and identify possible targets for adjuvant immunotherapy.
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| Overall design |
RNAsequencing profiles of PBMCs stimulated for 4h and 24h with live C. albicans and C. auris and their cell wall components
Treatment definitions:
RPMI: media control
calb_live : PBMCs stimulated with C. albicans live
calb_man: PBMCs stimulated with C. albicans-purified mannan
calb_bg: PBMCs stimulated with C. albicans-purified beta glucan
caur_KTClive: PBMCs stimulated with C. auris live
caur_KTCman: PBMCs stimulated with C. auris-purified mannan
caur_KTCbg: PBMCs stimulated with C. auris-purified beta glucan
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| Contributor(s) |
Bruno M, Kersten S, Hoischen A, Netea M |
| Citation(s) |
32839538 |
| |
| Submission date |
Jul 22, 2020 |
| Last update date |
Oct 22, 2020 |
| Contact name |
Mariolina Bruno |
| Organization name |
Radboudumc
|
| Department |
Experimental Internal Medicine
|
| Street address |
Geert Grooteplein Zuid 10
|
| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA647871 |
| SRA |
SRP273115 |
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