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| Status |
Public on Oct 29, 2020 |
| Title |
A distinct CD38+CD45RA+ population of CD4+, CD8+ and double-negative T cells is controlled by FAS (II) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The identification and characterization of rare immune cell populations in humans can be facilitated by their growth advantage in the context of specific genetic diseases. Here, we use autoimmune-lymphoproliferative syndrome to identify a population of FAS-controlled TCRab+ T cells. They include CD4+, CD8+ and double-negative T cells and can be defined by a CD38+CD45RA+T-BET- expression pattern. These unconventional T cells are present in all healthy individuals, generated before birth, enriched in lymphoid tissue and do not expand during acute viral infection. They are characterized by a unique molecular signature that is unambiguously different from other known T-cell differentiation subsets and independent of CD4 or CD8 expression. Functionally, FAS-controlled T cells represent highly proliferative, non-cytotoxic T cells with IL-10 cytokine bias. Mechanistically, regulation of this physiologic population is mediated by FAS and CTLA4 signaling and its survival is enhanced by mTOR and STAT3 signals. Genetic alterations in these pathways result in expansion of FAS-controlled T cells, which can cause significant lymphoproliferative disease.
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| Overall design |
Defined T cell subsets were FACS sorted from 3 ALPS-FAS patients and from 3 control donors for reference, replicates were not included
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| Contributor(s) |
Maccari ME, Kury P, Fuchs S, Andrieux G, Ekici AB, Erxleben A, Backofen R, Boerries M, Ehl S, Rensing-Ehl A |
| Citation(s) |
33170215 |
| Submission date |
Jul 22, 2020 |
| Last update date |
Nov 12, 2020 |
| Contact name |
Geoffroy Andrieux |
| Organization name |
University clinics Freiburg
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| Street address |
Breisacherstr 153
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| City |
Freiburg |
| ZIP/Postal code |
79110 |
| Country |
Germany |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (11)
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| GSM4683955 |
patient 6 FC DNT (CD3+TCRab+CD4-CD8-CD38+CD45RA+) |
| GSM4683956 |
patient 3 FC DNT (CD3+TCRab+CD4-CD8-CD38+CD45RA+) |
| GSM4683957 |
patient 22 FC DNT (CD3+TCRab+CD4-CD8-CD38+CD45RA+) |
| GSM4683958 |
HD4 FC DNT (CD3+TCRab+CD4-CD8-CD38+CD45RA+) |
| GSM4683959 |
HD5* FC DNT pooled with FC DNT from 3 additional HD (CD3+TCRab+CD4-CD8-CD38+CD45RA+) |
| GSM4683960 |
HD4 cDNT (CD3+TCRab+CD4-CD8- NOT CD38+CD45RA+) |
| GSM4683961 |
HD5 cDNT (CD3+TCRab+CD4-CD8- NOT CD38+CD45RA+) |
| GSM4683962 |
HD6 cDNT (CD3+TCRab+CD4-CD8- NOT CD38+CD45RA+) |
| GSM4683963 |
HD4 CD28+CD57-CD8+ N+ED (naive and early differentiated) |
| GSM4683964 |
HD5 CD28+CD57-CD8+ N+ED (naive and early differentiated) |
| GSM4683965 |
HD6 CD28+CD57-CD8+ N+ED (naive and early differentiated) |
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| This SubSeries is part of SuperSeries: |
| GSE154929 |
A distinct CD38+CD45RA+ population of CD4+, CD8+ and double-negative T cells is controlled by FAS |
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| Relations |
| BioProject |
PRJNA647893 |
| SRA |
SRP273130 |
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