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| Status |
Public on Jun 09, 2021 |
| Title |
Transcriptomic profiling of dimorphic growth in two Ustilaginomycotina species |
| Organisms |
Tilletiopsis washingtonensis; Meira miltonrushii |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we performed comparative transcriptomics to understand how mechanisms for fungal dimorphism are similar to the model species Ustilago maydis. To achieve this, we selected two non-model species from Ustilaginomycotina, Meira miltonrushii and Tilletiopsis washingtonensis, for transcriptomic profiling. Different culture conditions were used to have these two fungi display yeast growth (Holliday's minimal media with 1% glucose as a carbon source) and filamentous growth (Holliday's minimal media with 1% Tween as a carbon source). Then, we performed transcriptomic sequencing and analyses to determine which genes are differentially expressed in filamentous growth compared to yeast growth. We also incorporated public datasets from other two dimorphic species, U. maydis and Ophiostoma novo-ulmi, for comparison. We found that T. washingtonensis, despite being closely related to M. miltonrushii, is the most distant species in terms of the similarities of transcriptomic alteration, suggesting phylogenetic relatedness may not be concurrent with transcriptomic similarity under the same biological phenomenon. Functional enrichment analyses reveal that genes in cell energy production and conversion, amino acid transport and metabolism and cytoskeleton are groups of genes differentially expressed in common among the four species. Finally, although our comparative approach allows us to discover several candidate genes that may be involved in fungal dimorphism, we found very few commonly expressed genes that are know dimorphism genes characterized in U. maydis literature. This suggests the certain degree of species-specific mechanisms for dimorphic transition.
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| Overall design |
Examination of transcriptomic profiles under two growth forms (yeast and filamentous growth) in two fungal species (Tilletiopsis washingtonensis and Meira miltonrushii).
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| Contributor(s) |
Kijpornyongpan T, Aime MC |
| Citation(s) |
33991665 |
| |
| Submission date |
Jul 22, 2020 |
| Last update date |
Jun 09, 2021 |
| Contact name |
Teeratas Kijpornyongpan |
| E-mail(s) |
tkijporn@purdue.edu
|
| Phone |
7654763513
|
| Organization name |
Purdue University
|
| Department |
Botany and Plant Pathology
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| Lab |
Aime
|
| Street address |
915 W State St
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
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| Platforms (2) |
| GPL28907 |
Illumina NovaSeq 6000 (Tilletiopsis washingtonensis) |
| GPL28908 |
Illumina NovaSeq 6000 (Meira miltonrushii) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA647940 |
| SRA |
SRP273185 |
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