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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 24, 2023 |
| Title |
Expression data from human Dendritic cells (DCs) |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
microRNAs are crucial post-transcriptional regulators which contribute to the effect of sulforaphane in cancer and are also involved in the modulation of of many aspects of DC biology, including development, maturation, surface molecule expression and released cytokines
We used microarrays to detail the regulation of miRNAs in human DCs by sulforaphane, underlying the regulation of different crucial DCs regulatory molecules and signaling pathways
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| Overall design |
human monocyte-derived DCs (MoDCs) were produced, treated as described and used for miRNA extraction and hybridization on Affymetrix GeneChip miRNA 4.0 Arrays. We sought to increase the quality of produced MoDCs by isolation of initial Monocytes by CD14 MACS microbeads. The high quality MoDCs were proved by flow cytometry, pretreated with 30 mM sulforaphane (SF) for 30 min, treated with maturation cytokine cocktail or left untreated and collected for miRNA isolation after 48 hours incubation
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| Contributor(s) |
Wang Y, Karakhanova S |
| Citation(s) |
36569906 |
| Submission date |
Jul 28, 2020 |
| Last update date |
Jul 25, 2023 |
| Contact name |
Carolina Delatorre |
| E-mail(s) |
carolina.delatorre@medma.uni-heidelberg.de
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| Organization name |
University Heidelberg
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| Street address |
Theodor-Kutzer-Ufer 1-3
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| City |
Mannheim |
| ZIP/Postal code |
68167 |
| Country |
Germany |
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| Platforms (1) |
| GPL21572 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array [ProbeSet ID version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA649123 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155246_RAW.tar |
8.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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