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| Status |
Public on Nov 01, 2021 |
| Title |
Identification of viral and host targets of miR-138 by PAR-CLIP analysis of herpes simplex virus 2 infected 293T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Like herpes simplex virus 1 (HSV-1) ICP0 mRNA, HSV-2 ICP0 mRNA is predicted to be targeted by a host neuron-specific microRNA, miR-138. This study was designed to confirm the interaction between HSV-2 ICP0 mRNA and miR-138, and to identify other potential viral and host targets of miR-138 during HSV-2 infection. We performed PAR-CLIP on HSV-2 infected 293T cells overexpressing miR-138 in comparisin to control 293T cells. The results confirmed ICP0 as miR-138's targets, and also identified some other potential viral and host targets of miR-138.
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| Overall design |
Using previously generated 293T cells transduced with lentivirus expressing miR-138 and control 293T cells transduced with an empty vector, we infected these cells with HSV-2 for 4 hours at a multiplification of infection (MOI) of 1, and then harvested the cells for PAR-CLIP analysis. Sites with differential reads between the two kinds of cells are identified as potential target sites of miR-138.
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| Contributor(s) |
Pan D, Coen DM |
| Citation missing |
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| Submission date |
Jul 29, 2020 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Dongli Pan |
| E-mail(s) |
pandongli@zju.edu.cn
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| Organization name |
Zhejiang University
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| Street address |
866 Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
110058 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA649581 |
| SRA |
SRP274169 |
