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Series GSE155413 Query DataSets for GSE155413
Status Public on Feb 19, 2021
Title MeRIP-seq mapping of m6A in the IFN-β induced transcriptome in Huh7 cells
Organism Homo sapiens
Experiment type Other
Summary The RNA modification N6-methyladenosine (m6A) regulates gene expression through various transcript-specific effects. The goal of this experiment was to identify m6A-modified transcripts during the type I interferon response, with interest in determining which interferon-stimulated genes are modified by m6A. We found that a high percentage of ISGs are m6A-modified, and in follow-up experiments we determined the effects of m6A on the expression of interferon-stimulated genes. We found that m6A regulates the translation of a subset of ISGs, which in turn augments the antiviral effects of the type I interferon respnose.
 
Overall design Huh7 cells were seeded in 15-cm plates and treated with IFN-β at 50 units/mL or mock treated for 8 hours prior to harvest and MeRIP-seq was performed. 3 biological replicates were performed for each condition.
 
Contributor(s) McFadden M, McIntyre A, Gokhale N, Mason C, Horner S
Citation(s) 33657363
Submission date Jul 30, 2020
Last update date Apr 06, 2021
Contact name Christopher E Mason
E-mail(s) chm2042@med.cornell.edu
Organization name Weill Cornell Medical College
Department Physiology and Biophysics
Lab Christopher Mason Lab
Street address 1305 York Ave
City New York
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (12)
GSM4704026 Ifn input rep1
GSM4704027 Ifn input rep2
GSM4704028 Ifn input rep3
This SubSeries is part of SuperSeries:
GSE155448 Interferon response in Huh7 cells
Relations
BioProject PRJNA649691
SRA SRP274283

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE155413_Table_S2.1_meRIPseq.txt.gz 930.9 Kb (ftp)(http) TXT
GSE155413_Table_S2.2_meRIPseq_MeTDiff_peaks.txt.gz 602.7 Kb (ftp)(http) TXT
GSE155413_Table_S2.3_meRIPseq_meRIPPer_peaks.txt.gz 314.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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