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Status |
Public on Feb 19, 2021 |
Title |
MeRIP-seq mapping of m6A in the IFN-β induced transcriptome in Huh7 cells |
Organism |
Homo sapiens |
Experiment type |
Other
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Summary |
The RNA modification N6-methyladenosine (m6A) regulates gene expression through various transcript-specific effects. The goal of this experiment was to identify m6A-modified transcripts during the type I interferon response, with interest in determining which interferon-stimulated genes are modified by m6A. We found that a high percentage of ISGs are m6A-modified, and in follow-up experiments we determined the effects of m6A on the expression of interferon-stimulated genes. We found that m6A regulates the translation of a subset of ISGs, which in turn augments the antiviral effects of the type I interferon respnose.
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Overall design |
Huh7 cells were seeded in 15-cm plates and treated with IFN-β at 50 units/mL or mock treated for 8 hours prior to harvest and MeRIP-seq was performed. 3 biological replicates were performed for each condition.
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Contributor(s) |
McFadden M, McIntyre A, Gokhale N, Mason C, Horner S |
Citation(s) |
33657363 |
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Submission date |
Jul 30, 2020 |
Last update date |
Apr 06, 2021 |
Contact name |
Christopher E Mason |
E-mail(s) |
chm2042@med.cornell.edu
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Organization name |
Weill Cornell Medical College
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Department |
Physiology and Biophysics
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Lab |
Christopher Mason Lab
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Street address |
1305 York Ave
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City |
New York |
ZIP/Postal code |
10065 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (12)
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This SubSeries is part of SuperSeries: |
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Relations |
BioProject |
PRJNA649691 |
SRA |
SRP274283 |