|
Status |
Public on Jul 28, 2023 |
Title |
A regulatory circuit for maintenance and regeneration of root patterning |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
|
Summary |
We directed expression of BLUEJAY, JACKDAW and SCARECROW through the UAS promoter in the J0571 line and performed cell sorting followed by microarray experiments to identify differentially expressed genes.
|
|
|
Overall design |
Three independent replicates were processed for each control (J0571) and experimental (BLJ-J0571, JKD-J0571 and SCR-J0571). Seedlings were grown for 5 days and root tips were collected. J0571 and J0571-UAS-BIRDS sorted fluorecent cells were harvested for RNA extraction.
|
|
|
Contributor(s) |
Moreno-Risueno M, Sozzani R, Benfey PN |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Jul 30, 2020 |
Last update date |
Jul 28, 2023 |
Contact name |
Miguel Angel Moreno-Risueno |
E-mail(s) |
miguelangel.moreno@upm.es
|
Organization name |
Center for Plant Biotechnology and Genomics
|
Department |
Biotechnology
|
Lab |
Specification of new stem cells
|
Street address |
C/M-40 s/n
|
City |
Pozuelo de Alarcon |
State/province |
Madrid |
ZIP/Postal code |
28223 |
Country |
Spain |
|
|
Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
|
Samples (12)
|
GSM4704448 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_first biological replicate |
GSM4704449 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_second biological replicate |
GSM4704450 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_third biological replicate |
GSM4704451 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving BLJ_first biological replicate |
GSM4704452 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving BLJ_second biological replicate |
GSM4704453 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving BLJ_third biological replicate |
GSM4704454 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving JKD_first biological replicate |
GSM4704455 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving JKD_second biological replicate |
GSM4704456 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving JKD_third biological replicate |
GSM4704457 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving SCR_first biological replicate |
GSM4704458 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving SCR_second biological replicate |
GSM4704459 |
Arabidopsis ATH1_ground tissue marker_WT genetic background_UAS driving SCR_third biological replicate |
|
Relations |
BioProject |
PRJNA649765 |