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Status |
Public on Dec 31, 2020 |
Title |
Adeno-associated virus-mediated delivery of anti-miR-199a tough decoys attenuates cardiac hypertrophy |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
MicroRNAs (miRNAs) are important regulators in the process of cardiac hypertrophy and heart failure. Previous studies showed that miR-199a is upregulated in pressure-overload cardiac hypertrophy and overexpression of miR-199a induces cardiac hypertrophy in vivo. However, the therapeutic role of anti-miR-199a treatment in cardiac hypertrophy is of little known. Here, we showed a novel and effective way to treat mice cardiac hypertrophy and restored cardiac function through injection of adeno-associated virus (AAV) which expressed anti-miR-199a tough decoys (TuDs). We performed the RNA-seq profiling in both AAV9-EGFP control and AAV9-anti-miR-199a TuDs injected cardiac hypertrophic mice. The transcriptome analysis indicates that genes related to cytoplasmic translation, mitochondrial respiratory chain complex assembly were upregulated by anti-miR-199a treated recovered hearts.
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Overall design |
Examine expression of mRNAs in AAV9-EGFP and AAV9-anti-miR-199a tough decoys(TuDs) injected hypertrophic hearts
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Contributor(s) |
Yan H, Li Y, Hua Y, Luo Y |
Citation(s) |
33473326 |
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Submission date |
Aug 02, 2020 |
Last update date |
Jan 28, 2021 |
Contact name |
Hualin Yan |
E-mail(s) |
hualin.yan@scu.edu.cn
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Phone |
86-82-85423193
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Organization name |
West China Hospital, Sichuan University
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Street address |
No.37, Guoxuexiang,
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City |
Chengdu |
State/province |
Sichuan |
ZIP/Postal code |
610041 |
Country |
China |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA650214 |
SRA |
SRP275508 |