|
| Status |
Public on Aug 04, 2020 |
| Title |
Genome-scale mapping reveals complex regulatory activities of RpoN in Yersinia pseudotuberculosis [RNA-seq] |
| Organism |
Yersinia pseudotuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We combined phenotypic and genomic assays to provide insight into its role in this pathogen. RpoN was essential for Y. pseudotuberculosis virulence in mice, and in vitro functional assays showed that it controls biofilm formation and motility.
Mapping of genome-wide associations of Y. pseudotuberculosis RpoN identified an RpoN-binding motif at 103 inter- and intragenic sites located on both the sense and anti-sense strands
Transcriptomic analysis of bacteria lacking rpoN showed a large impact on gene expression, including down-regulation of genes encoding proteins involved in flagellar assembly, chemotaxis, and quorum sensing
There were also clear indications of cross talk with other sigma factors, together with indirect effects due to altered expression of other regulators. Matching differential gene expression with peak binding locations implicated around 130 genes or operons potentially activated or repressed by RpoN
|
| |
|
| Overall design |
RNA-seq of WT and RponN mutant in stationary and induction conditions.
|
| |
|
| Contributor(s) |
Firoj Mahmud AK, Nilsson K, Choudhury R, Fållman M |
| Citation(s) |
33172972 |
| |
| Submission date |
Aug 03, 2020 |
| Last update date |
Nov 24, 2020 |
| Contact name |
Maria Fallman |
| Organization name |
Umea university
|
| Department |
Molecular Biology
|
| Lab |
Fallman lab
|
| Street address |
Petrus Laestadius väg
|
| City |
Umea |
| ZIP/Postal code |
907 36 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL28960 |
Illumina MiSeq (Yersinia pseudotuberculosis) |
|
| Samples (12)
|
|
| This SubSeries is part of SuperSeries: |
| GSE155608 |
Genome-scale mapping reveals complex regulatory activities of RpoN in Yersinia pseudotuberculosis |
|
| Relations |
| BioProject |
PRJNA650494 |
| SRA |
SRP275637 |
Less...
More...