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Series GSE155612 Query DataSets for GSE155612
Status Public on Nov 13, 2020
Title The effects of METTL3 on islet β-cell function
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The goal of this study is to investigate how METTL3 regulates islet β-cell function.
Methods: Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from pancreatic islets of Mettl3flox/flox and β-Mettl3-KO mice at 8 weeks old. Each RNA sample was pooled from four Mettl3flox/flox and β-Mettl3-KO mice, respectively. Three independent biological replicates for each group were used for RNA-seq. RNA-seq was performed by deep sequencing using an Illumina Novaseq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(GRCm38.p6) with Hisat2 v2.0.5, and the aligned reads were used to quantify mRNA expression by using featureCounts v1.5.0-p3.
Conclusion: Our study represents the first detailed analysis of islet transcriptomes from Mettl3flox/flox and β-Mettl3-KO mice, generated by RNA-seq technology. The RNA-seq analysis showed that 2560 genes were downregulated and 3408 genes were upregulated in the pancreatic islets of β-Mettl3-KO mice. GO analysis showed that the downregulated genes were primarily related to insulin secretion, SNARE binding, and mitochondrial respiratory chain, whereas the upregulated genes were associated with the immune response, B cell activation, and antigen binding.
 
Overall design mRNA profiles in pancreatic islets of Mettl3flox/flox and β-Mettl3-KO mice were generated by deep sequencing using Illumina Novaseq 6000 platform (n=3 for each group).
 
Contributor(s) Chen Z, Li X
Citation(s) 33417895
Submission date Aug 03, 2020
Last update date Feb 13, 2021
Contact name Zheng Chen
E-mail(s) chenzheng@hit.edu.cn
Phone +8645186402029
Organization name Harbin Institute of Technology
Street address 92 Xidazhijie Harbin Institute of Technology
City Harbin
State/province Heilongjiang
ZIP/Postal code 150001
Country China
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM4708547 FF 1
GSM4708548 FF 2
GSM4708549 FF 3
Relations
BioProject PRJNA650497
SRA SRP275642

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Supplementary file Size Download File type/resource
GSE155612_gene_fpkm.txt.gz 2.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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