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Status |
Public on Nov 13, 2020 |
Title |
The effects of METTL3 on islet β-cell function |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: The goal of this study is to investigate how METTL3 regulates islet β-cell function. Methods: Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from pancreatic islets of Mettl3flox/flox and β-Mettl3-KO mice at 8 weeks old. Each RNA sample was pooled from four Mettl3flox/flox and β-Mettl3-KO mice, respectively. Three independent biological replicates for each group were used for RNA-seq. RNA-seq was performed by deep sequencing using an Illumina Novaseq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(GRCm38.p6) with Hisat2 v2.0.5, and the aligned reads were used to quantify mRNA expression by using featureCounts v1.5.0-p3. Conclusion: Our study represents the first detailed analysis of islet transcriptomes from Mettl3flox/flox and β-Mettl3-KO mice, generated by RNA-seq technology. The RNA-seq analysis showed that 2560 genes were downregulated and 3408 genes were upregulated in the pancreatic islets of β-Mettl3-KO mice. GO analysis showed that the downregulated genes were primarily related to insulin secretion, SNARE binding, and mitochondrial respiratory chain, whereas the upregulated genes were associated with the immune response, B cell activation, and antigen binding.
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Overall design |
mRNA profiles in pancreatic islets of Mettl3flox/flox and β-Mettl3-KO mice were generated by deep sequencing using Illumina Novaseq 6000 platform (n=3 for each group).
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Contributor(s) |
Chen Z, Li X |
Citation(s) |
33417895 |
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Submission date |
Aug 03, 2020 |
Last update date |
Feb 13, 2021 |
Contact name |
Zheng Chen |
E-mail(s) |
chenzheng@hit.edu.cn
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Phone |
+8645186402029
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Organization name |
Harbin Institute of Technology
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Street address |
92 Xidazhijie Harbin Institute of Technology
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City |
Harbin |
State/province |
Heilongjiang |
ZIP/Postal code |
150001 |
Country |
China |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA650497 |
SRA |
SRP275642 |