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| Status |
Public on Aug 04, 2020 |
| Title |
Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum |
| Organism |
Tribolium castaneum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In this study, RNA interference (RNAi) was conducted to investigate the effects of knockdown of TcAMPKα on lipid and carbohydrate metabolism in the red flour beetle, Tribolium castaneum, and the transcriptome profiles of dsTcAMPKα-injected and dsEGFP-injected beetles under normal conditions were compared by RNA-sequencing. Results: RNAi-mediated suppression of TcAMPKα increased whole-body triglyceride (TG) level and the ratio between glucose and trehalose, as was confirmed by in vivo treatment with the AMPK-activating compound, 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR). A total of 1184 differentially expressed genes (DEGs) were identified between dsTcAMPKα-injected and dsEGFP-injected beetles. These include genes involved in lipid and carbohydrate metabolism as well as insulin/insulin-like growth factor signaling (IIS). Real-time quantitative polymerase chain reaction analysis confirmed the differential expression of selected genes. Interestingly, metabolism-related transcription factors such as sterol regulatory element-binding protein 1 (SREBP1) and carbohydrate response element-binding protein (ChREBP) were also significantly upregulated in dsTcAMPKα-injected beetles. Conclusions: AMPK plays a critical role in the regulation of beetle metabolism. The findings of DEGs involved in lipid and carbohydrate metabolism provide valuable insight into the role of AMPK signaling in the transcriptional regulation of insect metabolism.
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| Overall design |
Total mRNA profiles of 20-day old dsEGFP-injected and dsTcAMPKα-injected larvae were generated by transcriptome sequencing under normal conditions with three replicates.
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| Contributor(s) |
Jiang H, Zhang N, Ji C, Meng X, Qian K, Zheng Y, Wang J |
| Citation(s) |
32967608 |
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| Submission date |
Aug 03, 2020 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Heng Jiang |
| E-mail(s) |
jiangheng9413@gmail.com
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| Phone |
+8618252711039
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| Organization name |
Yangzhou University
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| Street address |
88 University South Road
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| City |
Yangzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
225000 |
| Country |
China |
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| Platforms (1) |
| GPL18162 |
Illumina HiSeq 2500 (Tribolium castaneum) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA650526 |
| SRA |
SRP275683 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE155634_RAW.tar |
5.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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