|
| Status |
Public on Apr 11, 2009 |
| Title |
Identification of transcription factor DAF-16 binding sites in C. elegans |
| Project |
modENCODE
|
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We identified the DNA binding sites of DAF-16 in transgenic line TJ356 (daf-16::gfp) that carries high-copy number daf-16. The expression pattern was examined through fluorescent microscopy. The binding sites were determined using chromatin immunoprecipitation with anti-GFP antibody and anti-POL II antibody followed by illumina high-throughput sequencing (ChIP-seq).
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
|
| |
|
| Overall design |
Identification of DAF-16 binding sites at L4/Young adult stage when DAF-16 was highly overexpressed
|
| Web link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
|
| |
|
| Contributor(s) |
Zhong M, Niu W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA63461 |
| |
| Submission date |
Apr 06, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Flora Vaccarino |
| Organization name |
Yale University
|
| Department |
Child Study Center
|
| Lab |
Vaccarino
|
| Street address |
230 South Frontage Road
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9309 |
Illumina Genome Analyzer (Caenorhabditis elegans) |
|
| Samples (6)
|
| GSM389646 |
DAF-16 L4/Young adult replicate 1 GFP |
| GSM389647 |
DAF-16 L4/Young adult replicate 1 POL II |
| GSM389648 |
DAF-16 L4/Young adult replicate 1 Input |
| GSM389649 |
DAF-16 L4/Young adult replicate 2 GFP |
| GSM389650 |
DAF-16 L4/Young adult replicate 2 POL II |
| GSM389651 |
DAF-16 L4/Young adult replicate 2 Input |
|
| Relations |
| SRA |
SRP000864 |
Less...
More...