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| Status |
Public on Nov 28, 2013 |
| Title |
Gene expression in rectal epithelia of cystic fibrosis patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiles were recorded from rectal suction specimens of Cystic Fibrosis (CF) patients, carrying the CF-specific D508 mutated CFTR-allele. These profiles were compared with gene expression profiles from rectal suction specimens of non-CF subjects (control).
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| Overall design |
We used Affymetrix GeneChip HG-U133A microarrays to record the gene expression profile of 16 CF and 13 non-CF individuals.
Rectal suction specimens representing rectal mucosal epithelia were collected from CF patients and non-CF individuals. Biopsies were analyzed for residual function using ICM and total RNA was isolation immediately using the Qiagen RNeasy protocol with on column DNA digestion. About 1-5 µg RNA were obtained from each individual. Integrity of total RNA samples was monitored by gel-electrophoresis before cDNA synthesis. Labeled cRNA synthesis and hybridization was performed following standard protocols.
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| Contributor(s) |
Wölfl S, Pusch L, Kroll TC, van Barneveld A, Stanke F, Tamm S, Siebert B, Ballmann M, Junge S, Tümmler B |
| Citation(s) |
24105369, 21731057, 34680949 |
| Submission date |
Apr 06, 2009 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Stefan Wolfl |
| E-mail(s) |
wolfl@uni-hd.de
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| Organization name |
Heidelberg University, Heidelberg, Germany
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| Department |
Institute of Pharmacy and Molecular Biotechnology
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| Lab |
Pharm. Biology and Bioanalytics
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| Street address |
Im Neuenheimer Feld 364
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| City |
Heidelberg |
| ZIP/Postal code |
D-69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA115739 |
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