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| Status |
Public on Aug 04, 2022 |
| Title |
HDAC inhibition suppresses telomerase activity in neuroblastoma cells harboring TERT rearrangements [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. The three histone marks acetylation of histone 3 at lysine residue 27 (H3K27ac), trimethylation of histone 3 at lysine residue 4 (H3K4me3) and trimethylation of histone 3 at lysine residue 36 (H3K36me3) were selected, representing marks for an open chromatin structure and enable gene transcription. The two histone marks standing for a condensed chromatin state and disabled gene transcription trimethylation of histone 3 at lysine residue 27 (H3K27me3) and trimethylation of histone 3 at lysine residue 9 (H3K9me3) were chosen. ChIP sequencing of the five histone marks was investigated in high-risk TERT-rearranged neuroblastoma GI-ME-N cells treated with solvent or HDAC inhibitor panobinostat. Exemplary samples include solvent-treated and panobinostat-treated samples after 18 h of treatment.
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| Overall design |
Characterization of histone modifications in the TERT-rearranged neuroblastoma cell line GI-ME-N treated with panobinostat or solvent control
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| Contributor(s) |
Finkler S, Weiner J, Helmsauer K, Sprüssel A, Arlt B, Kuenkele A, Schulte JH, Haase K, Obermayer B, Beule D, Fischer M, Bluethgen N, Henssen AG, Lodrini M, Eggert A, Deubzer HE |
| Citation missing |
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| |
| Submission date |
Aug 04, 2020 |
| Last update date |
Jan 06, 2023 |
| Contact name |
January 3rd Weiner |
| E-mail(s) |
january.weiner@gmail.com
|
| Phone |
030450543049
|
| Organization name |
Berlin Institute of Health, Charité Medical University of Berlin
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| Department |
CUBI
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (40)
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| This SubSeries is part of SuperSeries: |
| GSE155707 |
HDAC inhibition suppresses telomerase activity in neuroblastoma cells harboring TERT rearrangements |
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| Relations |
| BioProject |
PRJNA655249 |
| SRA |
SRP275845 |
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