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| Status |
Public on Aug 05, 2023 |
| Title |
Identification of genes differentially expressed between the prostates of PtenPC+/- mice and the prostate tumors of PtenPC+/-;Ncoa6PC-/- mice at the age of 9 months |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we aimed to characterize the suppressive role of NCOA6 in prostate cancer development, uncover the underlying molecular mechanism, and identify potential therapeutic targets for treating NCOA6 loss-induced prostate cancer. Gene expression microarray analysis and RNA-Seq analysis were performed to identify differentially expressed genes influenced by NCOA6 in 22Rv1 human prostate cancer cells and mouse prostate tumors, respectively. Pathway enrichment analysis and gene set enrichment analysis were performed to identify NCOA6-regulated signaling pathways, particually the cancer-associated pathways. ChIP-Seq analysis was further performed to identify NCOA6-associated genomic regions in 22Rv1 cells. The potential direct target genes of NCOA6 were identified by combined analysis of the gene expression profiling data and ChIP-Seq data. Finally, we identified EGFR as one of the 264 NCOA6 direct target genes and deomonstrated that NCOA6 suppressed prostate cancer progression by preventing EGFR overexpression.
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| Overall design |
Gene expression profiles in the prostates of PtenPC+/- mice and the prostate tumors of PtenPC+/-;Ncoa6PC-/- mice at the age of 9 months were examined.
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| Contributor(s) |
Liu Y, Xu J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 05, 2020 |
| Last update date |
Aug 05, 2023 |
| Contact name |
Yonghong Liu |
| E-mail(s) |
yonghongliu2017@gmail.com
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| Phone |
7137984684
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| Organization name |
Baylor College of Medicne
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| Department |
Molecular & Cellular Biology
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| Lab |
N630
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| Street address |
One Baylor Plaza
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE155757 |
NCOA6 is a potent prostate cancer suppressor essential for NFY-mediated restriction of EGFR overexpression |
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| Relations |
| BioProject |
PRJNA655485 |
| SRA |
SRP276118 |
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