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Status |
Public on Nov 05, 2020 |
Title |
Genome-wide expression profiling of acute promyelocytic leukemia NB4 cells treated with all-trans-retinoic acid |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Acute promyelocytic leukemia (APL) is a hematological disease characterized by a balanced reciprocal translocation that leads to the synthesis of the oncogenic fusion protein PML-RARα. APL is mainly managed by a differentiation therapy based on the administration of all-trans retinoic acid (ATRA) and arsenic trioxide (ATO). However, therapy resistance, differentiation syndrome, and relapses require the development of new low-toxicity therapies based on the induction of blasts differentiation. Here, we performed a high-throughput gene expression profile of the maturation inducible APL cell line NB4 untreated or exposed to ATRA.
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Overall design |
We performed a whole mRNA transcriptome analysis by Affymetrix microarray of NB4 cells exposed to 1 µM ATRA for 120h or only to DMSO as control (Ctrl). Two biological replicates were performed for each condition.
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Contributor(s) |
Bianchi F, Di Masi A |
Citation(s) |
33167477 |
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Submission date |
Aug 05, 2020 |
Last update date |
Feb 04, 2021 |
Contact name |
Fabrizio Bianchi |
E-mail(s) |
f.bianchi@operapadrepio.it
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Organization name |
Fondazione IRCCS Casa Sollievo della Sofferenza
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Lab |
Cancer Biomarkers
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Street address |
Viale Padre Pio 7
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City |
San Giovanni Rotondo (FG) |
State/province |
Outside U.S. & Canada |
ZIP/Postal code |
71013 |
Country |
Italy |
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Platforms (1) |
GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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Samples (4)
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Relations |
BioProject |
PRJNA655564 |