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| Status |
Public on Dec 11, 2020 |
| Title |
RNA sequencing of tomato seed tissues during seed development |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During maturation seeds acquire several physiological traits to enable them to survive drying and disseminate the species. Few studies have addressed the regulatory networks controlling acquisition of these traits at the tissue level particularly in endospermic seeds such as tomato, which matures in a fully hydrated environment and does not undergo maturation drying. Using temporal RNA-seq analyses of the different seed tissues during maturation, gene network and trait-based correlations were used to explore the transcriptome signatures and identify hubs associated with desiccation tolerance, longevity, germination under water stress and dormancy.
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| Overall design |
Three replicates of 10-20 fresh tomato seeds were harvested at 14 stages throughout seed development from 15 DAF to overripened fruits (red plus 14 days (R14)). RNA-seq data series of whole seeds and isolated seed tissues was obtained from 15 to 28 DAF for entire seeds, from 35 DAF to stage R14 for embryo and endosperm, and from 35 to 49 DAF for seed coat.
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| Contributor(s) |
Bizouerne E, Leprince O |
| Citation(s) |
33648457, 37896095 |
| |
| Submission date |
Aug 06, 2020 |
| Last update date |
Nov 09, 2023 |
| Contact name |
Julia Buitink |
| E-mail(s) |
julia.buitink@inrae.fr
|
| Phone |
+33241225544
|
| Organization name |
INRAE
|
| Department |
BAP
|
| Lab |
SEED
|
| Street address |
42 rue Georges Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49070 |
| Country |
France |
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| Platforms (2) |
| GPL26877 |
BGISEQ-500 (Solanum lycopersicum) |
| GPL28981 |
DNBSEQ-G400 (Solanum lycopersicum) |
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| Samples (84)
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| Relations |
| BioProject |
PRJNA655733 |
| SRA |
SRP276494 |
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