|
| Status |
Public on May 05, 2021 |
| Title |
LoopSeq Synthetic Long Read Sequencing Uncovers Isoform Modulation in the progression of Colon Cancer [Loop QC] |
| Organisms |
Homo sapiens; synthetic construct |
| Experiment type |
Other
|
| Summary |
In this study, we used a barcoding-based synthetic long read (SLR) isoform sequencing approach (LoopSeq) to generate sequencing reads sufficiently long and accurate to identify isoforms using standard short read Illumina sequencers.
|
| |
|
| Overall design |
Loop transcriptome sequencing on ERCC synthetic RNA (ThermoFisher Scientific) for quality control.
|
| |
|
| Contributor(s) |
Liu S, Wu I, Yu Y, Balamotis M, Ren B, Yehezkel TB, Luo J |
| Citation(s) |
33907296 |
| |
| Submission date |
Aug 08, 2020 |
| Last update date |
May 05, 2021 |
| Contact name |
Shuchang Liu |
| E-mail(s) |
shl96@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Street address |
203 Lothrop Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL23058 |
Illumina NextSeq 500 (Homo sapiens; synthetic construct) |
| GPL28989 |
Illumina HiSeq 4000 (Homo sapiens; synthetic construct) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE155921 |
LoopSeq Synthetic Long Read Sequencing Uncovers Isoform Modulation in the progression of Colon Cancer |
|
| Relations |
| BioProject |
PRJNA656369 |
| SRA |
SRP277085 |
