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Status |
Public on Aug 12, 2020 |
Title |
miRNA expression in tracheal aspirates |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by RT-PCR
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Summary |
Extreme preterm infants are a growing population in neonatal intensive care units who carry a high mortality and morbidity. Multiple factors play a role in preterm birth, resulting in major impact on organogenesis leading to complications including bronchopulmonary dysplasia (BPD). The goal of this study was to identify biomarker signatures associated with prematurity and BPD. We analyzed miRNA and mRNA profiles in tracheal aspirates (TAs) from 51 infants receiving invasive mechanical ventilation. 25 infants were extremely preterm and diagnosed with BPD, and 26 were term babies receiving invasive mechanical ventilation for elective procedures. We found specific mRNA-miRNA signatures in TAs that may serve as biomarkers for BPD pathogenesis, a consequence of extreme prematurity.
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Overall design |
We extracted RNA from 0.5 mL of tracheal aspirates from control neonates and premature babies with bronchopulmonary dysplasia using the Plasma/Serum RNA Purification Kit. The expression of 1,066 human miRNAs was quantified using the Human miRNome miScript® miRNA PCR Array (QIAGEN, MIHS-3216Z). Results (Ct values) were normalized to global means, and fold changes were calculated using the 2-ΔΔCT method. control: 2,6,7,10,11,14,17,18,23,24,26,28,30,32,33,34,36,38,40,41,42,43,44,46,49,50 BPD: 1,3,4,5,8,9,12,13,15,16,19,20,21,22,25,27,29,31,35,37,39,45,47,48,51
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Contributor(s) |
Silveyra P, Siddaiah R, Oji-Mmuo C, Fuentes N, Montes D |
Citation(s) |
33177681 |
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Submission date |
Aug 11, 2020 |
Last update date |
Nov 12, 2020 |
Contact name |
Patricia Silveyra |
E-mail(s) |
psilveyr@iu.edu, ptrcslvr@gmail.com
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Organization name |
Indiana University Bloomington
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Department |
Environmental and Occupational Health
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Lab |
Silveyra lab
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Street address |
2819 E 10th St
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City |
Bloomington |
State/province |
Indiana |
ZIP/Postal code |
47408 |
Country |
USA |
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Platforms (1) |
GPL26552 |
Qiagen Human miRNome miScript miRNA PCR Array (MIHS-3216Z) |
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Samples (51)
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GSM4721430 |
BPD, Sample 4 |
GSM4721431 |
BPD, Sample 5 |
GSM4721432 |
Control, Sample 6 |
GSM4721433 |
Control, Sample 7 |
GSM4721434 |
BPD, Sample 8 |
GSM4721435 |
BPD, Sample 9 |
GSM4721436 |
Control, Sample 10 |
GSM4721437 |
Control, Sample 11 |
GSM4721438 |
BPD, Sample 12 |
GSM4721439 |
BPD, Sample 13 |
GSM4721440 |
Control, Sample 14 |
GSM4721441 |
BPD, Sample 15 |
GSM4721442 |
BPD, Sample 16 |
GSM4721443 |
Control, Sample 17 |
GSM4721444 |
Control, Sample 18 |
GSM4721445 |
BPD, Sample 19 |
GSM4721446 |
BPD, Sample 20 |
GSM4721447 |
BPD, Sample 21 |
GSM4721448 |
BPD, Sample 22 |
GSM4721449 |
Control, Sample 23 |
GSM4721450 |
Control, Sample 24 |
GSM4721451 |
BPD, Sample 25 |
GSM4721452 |
Control, Sample 26 |
GSM4721453 |
BPD, Sample 27 |
GSM4721454 |
Control, Sample 28 |
GSM4721455 |
BPD, Sample 29 |
GSM4721456 |
Control, Sample 30 |
GSM4721457 |
BPD, Sample 31 |
GSM4721458 |
Control, Sample 32 |
GSM4721459 |
Control, Sample 33 |
GSM4721460 |
Control, Sample 34 |
GSM4721461 |
BPD, Sample 35 |
GSM4721462 |
Control, Sample 36 |
GSM4721463 |
BPD, Sample 37 |
GSM4721464 |
Control, Sample 38 |
GSM4721465 |
BPD, Sample 39 |
GSM4721466 |
Control, Sample 40 |
GSM4721467 |
Control, Sample 41 |
GSM4721468 |
Control, Sample 42 |
GSM4721469 |
Control, Sample 43 |
GSM4721470 |
Control, Sample 44 |
GSM4721471 |
BPD, Sample 45 |
GSM4721472 |
Control, Sample 46 |
GSM4721473 |
BPD, Sample 47 |
GSM4721474 |
BPD, Sample 48 |
GSM4721475 |
Control, Sample 49 |
GSM4721476 |
Control, Sample 50 |
GSM4721477 |
BPD, Sample 51 |
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Relations |
BioProject |
PRJNA656545 |
Supplementary file |
Size |
Download |
File type/resource |
GSE156055_RAW.tar |
30.0 Kb |
(http)(custom) |
TAR |
GSE156055_fold-change_data.txt.gz |
208.2 Kb |
(ftp)(http) |
TXT |
GSE156055_non-normalized_data.txt.gz |
230.5 Kb |
(ftp)(http) |
TXT |
Processed data are available on Series record |
Processed data included within Sample table |
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