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Series GSE156055 Query DataSets for GSE156055
Status Public on Aug 12, 2020
Title miRNA expression in tracheal aspirates
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary Extreme preterm infants are a growing population in neonatal intensive care units who carry a high mortality and morbidity. Multiple factors play a role in preterm birth, resulting in major impact on organogenesis leading to complications including bronchopulmonary dysplasia (BPD). The goal of this study was to identify biomarker signatures associated with prematurity and BPD. We analyzed miRNA and mRNA profiles in tracheal aspirates (TAs) from 51 infants receiving invasive mechanical ventilation. 25 infants were extremely preterm and diagnosed with BPD, and 26 were term babies receiving invasive mechanical ventilation for elective procedures. We found specific mRNA-miRNA signatures in TAs that may serve as biomarkers for BPD pathogenesis, a consequence of extreme prematurity.
 
Overall design We extracted RNA from 0.5 mL of tracheal aspirates from control neonates and premature babies with bronchopulmonary dysplasia using the Plasma/Serum RNA Purification Kit. The expression of 1,066 human miRNAs was quantified using the Human miRNome miScript® miRNA PCR Array (QIAGEN, MIHS-3216Z). Results (Ct values) were normalized to global means, and fold changes were calculated using the 2-ΔΔCT method.
control: 2,6,7,10,11,14,17,18,23,24,26,28,30,32,33,34,36,38,40,41,42,43,44,46,49,50
BPD: 1,3,4,5,8,9,12,13,15,16,19,20,21,22,25,27,29,31,35,37,39,45,47,48,51
 
Contributor(s) Silveyra P, Siddaiah R, Oji-Mmuo C, Fuentes N, Montes D
Citation(s) 33177681
Submission date Aug 11, 2020
Last update date Nov 12, 2020
Contact name Patricia Silveyra
E-mail(s) psilveyr@iu.edu, ptrcslvr@gmail.com
Organization name Indiana University Bloomington
Department Environmental and Occupational Health
Lab Silveyra lab
Street address 2819 E 10th St
City Bloomington
State/province Indiana
ZIP/Postal code 47408
Country USA
 
Platforms (1)
GPL26552 Qiagen Human miRNome miScript miRNA PCR Array (MIHS-3216Z)
Samples (51)
GSM4721427 BPD, Sample 1
GSM4721428 Control, Sample 2
GSM4721429 BPD, Sample 3
Relations
BioProject PRJNA656545

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156055_RAW.tar 30.0 Kb (http)(custom) TAR
GSE156055_fold-change_data.txt.gz 208.2 Kb (ftp)(http) TXT
GSE156055_non-normalized_data.txt.gz 230.5 Kb (ftp)(http) TXT
Processed data are available on Series record
Processed data included within Sample table

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