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Series GSE156066 Query DataSets for GSE156066
Status Public on Feb 19, 2021
Title Evaluating the effect of spaceflight on the host-pathogen interaction between human intestinal epithelial cells and Salmonella Typhimurium
Organisms Homo sapiens; Salmonella enterica subsp. enterica serovar Typhimurium
Experiment type Expression profiling by high throughput sequencing
Summary Spaceflight uniquely alters the physiology of both human cells and microbial pathogens, stimulating cellular and molecular changes directly relevant to infectious disease. However, the influence of this environment on host-pathogen interactions remains poorly understood. Here we report our results from the STL-IMMUNE pilot study flown aboard STS-131, which investigated multi-omic responses (transcriptomic, proteomic) of human intestinal epithelial cells to infection with Salmonella Typhimurium when both host and pathogen were simultaneously exposed to spaceflight. To our knowledge, this is the first in vitro in-flight infection and dual RNA-seq analysis using human cells.
Overall design Human colonic epithelial cells were cultured in hollow fiber bioreactors in space aboard Space Shuttle Discovery (mission STS-131). Synchronous ground controls were performed in the same hardware on Earth at Kennedy Space Center. On Flight Day 11, Salmonella Typhimurium (~1x10^7 CFU) was injected into a subset of the cultures while the remaining cultures served as time-matched uninfected controls. Six hours post-infection, cultures were fixed in RNAlater II for post-flight analysis.
Contributor(s) Barrila J, Sarker SF, Hansmeier N, Yang S, Buss K, Briones N, Park J, Davis RR, Forsyth RJ, Ott C, Sato K, Kosnik C, Yang A, Shimoda C, Rayl N, Ly D, Landenberger A, Wilson SD, Yamazaki N, Steel J, Montano C, Halden RU, Cannon T, Castro-Wallace SL, Nickerson CA
Citation(s) 33750813
Submission date Aug 11, 2020
Last update date Apr 07, 2021
Contact name Jennifer Barrila
Phone 480-727-9282
Organization name Arizona State University
Department Biodesign Center for Fundamental and Applied Microbiomics
Street address 1001 S McAllister Ave
City Tempe
State/province AZ
ZIP/Postal code 85287
Country USA
Platforms (2)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL29006 Illumina NovaSeq 6000 (Homo sapiens; Salmonella enterica subsp. enterica serovar Typhimurium)
Samples (8)
GSM4721836 Flight Uninfected-1
GSM4721837 Flight Uninfected-2
GSM4721838 Flight Infected 1-1
BioProject PRJNA656571
SRA SRP277095

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Supplementary file Size Download File type/resource
GSE156066_annotated_fpkm_TPM.txt.gz 2.5 Mb (ftp)(http) TXT
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