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Series GSE156201 Query DataSets for GSE156201
Status Public on Jan 25, 2021
Title Single cell transcriptomic data from MGE-derived interneurons purified from postnatal day 18-21 frontal cortex and hippocampus of wild type and MGE-specific GRIN1-knockout mice.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Medial ganglionic eminence (MGE)-derived parvalbumin (PV)+, somatostatin (SST)+ and Neurogliaform (NGFC)-type cortical and hippocampal interneurons, have distinct molecular, anatomical and physiological properties. However, the molecular mechanisms regulating their diversity remain poorly understood. Here, via single-cell transcriptomics, we show that the obligate NMDA-type glutamate receptor (NMDAR) subunit gene Grin1 mediates subtype-specific transcriptional regulation of gene expression in MGE-derived interneurons, leading to altered subtype identities. Notably, MGE-specific conditional Grin1 loss results in a systemic downregulation of diverse transcriptional, synaptogenic and membrane excitability regulatory programs. These widespread gene expression abnormalities mirror aberrations that are typically associated with neurodevelopmental disorders, particularly schizophrenia. Our study hence provides a roadmap for the systematic examination of NMDAR signaling in interneuron subtypes, revealing potential MGE-specific genetic targets that could instruct future therapies of psychiatric disorders.
 
Overall design Frontal cortex and hippocampus of wild-type and MGE-specific GRIN1-knockout mice have been dissected and FACS-sorted Nkx2.1-cell-type specific cells (Td-tomato positive)
 
Contributor(s) Mahadevan V, Mitra A, Zhang Y, Peltekian A, Chittajallu R, Esnault C, Maric D, Rhodes C, Pelkey KA, Dale R, Petros TJ, McBain CJ
Citation(s)
  • Mahadevan V, Mitra A, Zhang Y, Yuan X et al. NMDARs Drive the Expression of Neuropsychiatric Disorder Risk Genes Within GABAergic Interneuron Subtypes in the Juvenile Brain. Front Mol Neurosci 2021;14:712609. PMID: 34630033
Submission date Aug 13, 2020
Last update date Oct 14, 2021
Contact name Vivek Mahadevan
E-mail(s) vivek.mahadevan@nih.gov
Organization name Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
Department aSection on Cellular and Synaptic Physiology
Lab Chris McBain Lab
Street address 35, Convent Drive
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM4726598 Frontal cortex cells in MGE-derived interneuron-specific GRIN1-KO background
GSM4726599 Frontal cortex cells in WT background
GSM4726600 Hippocampus cells in MGE-derived interneuron-specific GRIN1-KO background
Relations
BioProject PRJNA657037
SRA SRP277409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156201_RAW.tar 221.8 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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