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Status |
Public on Jan 25, 2021 |
Title |
Single cell transcriptomic data from MGE-derived interneurons purified from postnatal day 18-21 frontal cortex and hippocampus of wild type and MGE-specific GRIN1-knockout mice. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Medial ganglionic eminence (MGE)-derived parvalbumin (PV)+, somatostatin (SST)+ and Neurogliaform (NGFC)-type cortical and hippocampal interneurons, have distinct molecular, anatomical and physiological properties. However, the molecular mechanisms regulating their diversity remain poorly understood. Here, via single-cell transcriptomics, we show that the obligate NMDA-type glutamate receptor (NMDAR) subunit gene Grin1 mediates subtype-specific transcriptional regulation of gene expression in MGE-derived interneurons, leading to altered subtype identities. Notably, MGE-specific conditional Grin1 loss results in a systemic downregulation of diverse transcriptional, synaptogenic and membrane excitability regulatory programs. These widespread gene expression abnormalities mirror aberrations that are typically associated with neurodevelopmental disorders, particularly schizophrenia. Our study hence provides a roadmap for the systematic examination of NMDAR signaling in interneuron subtypes, revealing potential MGE-specific genetic targets that could instruct future therapies of psychiatric disorders.
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Overall design |
Frontal cortex and hippocampus of wild-type and MGE-specific GRIN1-knockout mice have been dissected and FACS-sorted Nkx2.1-cell-type specific cells (Td-tomato positive)
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Contributor(s) |
Mahadevan V, Mitra A, Zhang Y, Peltekian A, Chittajallu R, Esnault C, Maric D, Rhodes C, Pelkey KA, Dale R, Petros TJ, McBain CJ |
Citation(s) |
- Mahadevan V, Mitra A, Zhang Y, Yuan X et al. NMDARs Drive the Expression of Neuropsychiatric Disorder Risk Genes Within GABAergic Interneuron Subtypes in the Juvenile Brain. Front Mol Neurosci 2021;14:712609. PMID: 34630033
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Submission date |
Aug 13, 2020 |
Last update date |
Oct 14, 2021 |
Contact name |
Vivek Mahadevan |
E-mail(s) |
vivek.mahadevan@nih.gov
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Organization name |
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
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Department |
aSection on Cellular and Synaptic Physiology
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Lab |
Chris McBain Lab
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Street address |
35, Convent Drive
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City |
Bethesda |
State/province |
Maryland |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (4)
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GSM4726598 |
Frontal cortex cells in MGE-derived interneuron-specific GRIN1-KO background |
GSM4726599 |
Frontal cortex cells in WT background |
GSM4726600 |
Hippocampus cells in MGE-derived interneuron-specific GRIN1-KO background |
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Relations |
BioProject |
PRJNA657037 |
SRA |
SRP277409 |
Supplementary file |
Size |
Download |
File type/resource |
GSE156201_RAW.tar |
221.8 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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