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| Status |
Public on May 11, 2021 |
| Title |
Transcriptome Analysis of Non-Human Primate Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 2D Monolayer Culture versus 3D Engineered Heart Tissue |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we utilized both monolayer culture (2D) and engineered heart tissue (EHT) (3D) with NHP iPSC-CMs, which served as surrogates of direct cell injection and cell patch in vivo during exposure to hypoxic conditions, and the outcomes were compared at the transcriptome level. We found the 3D EHT model was more sensitive to ischemic conditions and similar to the native in vivo myocardium in terms of cell-extracellular matrix/cell-cell interactions, energy metabolism, and paracrine signaling.
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| Overall design |
6 samples for both monolayer culture (2D) and engineered heart tissue (EHT) (3D) with NHP iPSC-CMs, and 3 samples in each conditions were treated with exposure to hypoxic conditions or not.
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| Contributor(s) |
Yang H, Shao N, Wu J |
| Citation(s) |
33002105 |
| |
| Submission date |
Aug 14, 2020 |
| Last update date |
May 12, 2021 |
| Contact name |
Joseph Wu |
| Organization name |
Stanford University School of Medicine
|
| Street address |
Stanford University School of Medicine
|
| City |
Stanford |
| State/province |
Carlifornia |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL29020 |
Ion Torrent PGM (Macaca mulatta) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA657164 |
| SRA |
SRP277489 |
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