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Status |
Public on Aug 16, 2023 |
Title |
Activity of the GHRH Antagonist MIA-602 and its Underlying Mechanisms of Action in sarcoidosis |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Growth hormone releasing hormone (GHRH) is a potent stimulator of growth hormone secretion from pituitary gland. Although GHRH is essential for growth of immune cells, the regulatory effects of its antagonist in granulomatous disease remains unknown. The goal is to evaluate the expression of GHRH receptor in human tissue with sarcoidosis granuloma and demonstrate the anti-inflammatory effects of MIA-602 (a GHRH antagonist) in in vitro and in vivo studies. Granulomatous reaction in the mouse lung was developed as previously described (PMID: 31921151). Mice were treated with 5 micrograms of MIA-602 (an GHRH antagonist) daily subcutanously. RNA from mouse lungs were extracted and RNASeq was perfomed.
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Overall design |
We selected 3 mice for MIA 602 treatment. They all were challenged with microparticles generated from Mycobacterium abscessus for granuloma development. MIA-602 was given daily via under skin injection. Mice were sacrificed after 3 weeks and lungs were extracted for analysis.
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Contributor(s) |
Griswold AJ, Mirsaeidi M |
Citation missing |
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Submission date |
Aug 18, 2020 |
Last update date |
Aug 16, 2023 |
Contact name |
Anthony Griswold |
E-mail(s) |
agriswold@med.miami.edu
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Organization name |
University of Miami
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Street address |
1501 NW 10th Ave, BRB 318
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City |
Miami |
State/province |
FL |
ZIP/Postal code |
33136 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (3) |
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This SubSeries is part of SuperSeries: |
GSE140439 |
Mycobacterium abscessus -bronchial epithelial cells cross-talk through Type I interferon signaling |
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Relations |
BioProject |
PRJNA657823 |
SRA |
SRP277918 |