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Series GSE156397 Query DataSets for GSE156397
Status Public on Aug 16, 2023
Title Activity of the GHRH Antagonist MIA-602 and its Underlying Mechanisms of Action in sarcoidosis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Growth hormone releasing hormone (GHRH) is a potent stimulator of growth hormone secretion from pituitary gland. Although GHRH is essential for growth of immune cells, the regulatory effects of its antagonist in granulomatous disease remains unknown. The goal is to evaluate the expression of GHRH receptor in human tissue with sarcoidosis granuloma and demonstrate the anti-inflammatory effects of MIA-602 (a GHRH antagonist) in in vitro and in vivo studies. Granulomatous reaction in the mouse lung was developed as previously described (PMID: 31921151). Mice were treated with 5 micrograms of MIA-602 (an GHRH antagonist) daily subcutanously. RNA from mouse lungs were extracted and RNASeq was perfomed.
 
Overall design We selected 3 mice for MIA 602 treatment. They all were challenged with microparticles generated from Mycobacterium abscessus for granuloma development. MIA-602 was given daily via under skin injection. Mice were sacrificed after 3 weeks and lungs were extracted for analysis.
 
Contributor(s) Griswold AJ, Mirsaeidi M
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Submission date Aug 18, 2020
Last update date Aug 16, 2023
Contact name Anthony Griswold
E-mail(s) agriswold@med.miami.edu
Organization name University of Miami
Street address 1501 NW 10th Ave, BRB 318
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (3)
GSM4730146 MIA6024
GSM4730147 MIA6025
GSM4730148 MIA6026
This SubSeries is part of SuperSeries:
GSE140439 Mycobacterium abscessus -bronchial epithelial cells cross-talk through Type I interferon signaling
Relations
BioProject PRJNA657823
SRA SRP277918

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Supplementary file Size Download File type/resource
GSE156397_overall_counts.txt.gz 337.5 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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